ArticleGene therapy2007
Non-invasive in vivo optical imaging of the lacZ and luc gene expression in mice.
Article in Gene therapy, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed.
- Article
- In Vivo Evaluation of a Miniaturized Fluorescence Molecular Tomography (FMT) Endoscope for Breast Cancer Detection Using Targeted Nanoprobes.International journal of molecular sciences · 2020Article
- Bioprofiling TS/A Murine Mammary Cancer for a Functional Precision Experimental Model.Cancers · 2019Review
- Full density fluorescence molecular tomography (FD-FMT) based on a dichroic mirror.Applied optics · 2018Article
- MRI Reporter Genes for Noninvasive Molecular Imaging.Molecules (Basel, Switzerland) · 2016Review
- A lacZ Reporter-Based Strategy for Rapid Expression Analysis and Target Validation of Mycobacterium tuberculosis Latent Infection Genes.Current microbiology · 2016Article
- IPP51, a chalcone acting as a microtubule inhibitor with in vivo antitumor activity against bladder carcinoma.Oncotarget · 2015Article
- Sulfatase-activated fluorophores for rapid discrimination of mycobacterial species and strains.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Novel S-Gal(®) analogs as (1)H MRI reporters for in vivo detection of β-galactosidase.Magnetic resonance imaging · 2013Article
- The dual effect of mesenchymal stem cells on tumour growth and tumour angiogenesis.Stem cell research & therapy · 2013Article
- Near-infrared optical imaging of nucleic acid nanocarriers in vivo.Methods in molecular biology (Clifton, N.J.) · 2013Article
- Reduction of renal uptake of 111In-DOTA-labeled and A700-labeled RAFT-RGD during integrin αvβ3 targeting using single photon emission computed tomography and optical imaging.Cancer science · 2012Article
- Dual 19F/1H MR gene reporter molecules for in vivo detection of β-galactosidase.Bioconjugate chemistry · 2012Article
- Imaging gene expression in live cells and tissues.Cold Spring Harbor protocols · 2011Article
- Enhanced tumor therapy using vaccinia virus strain GLV-1h68 in combination with a β-galactosidase-activatable prodrug seco-analog of duocarmycin SA.Cancer gene therapy · 2011Article
- Ex vivo culture of patient tissue & examination of gene delivery.Journal of visualized experiments : JoVE · 2010Article
- Molecular imaging of in vivo gene expression.Future medicinal chemistry · 2010Review
- Targeting cyclin B1 through peptide-based delivery of siRNA prevents tumour growth.Nucleic acids research · 2009Article
- In Vivo Cellular Imaging for Translational Medical Research.Current medical imaging reviews · 2009Article
- Whole animal in vivo imaging after transient, nonviral gene delivery to the rat central nervous system.Molecular therapy : the journal of the American Society of Gene Therapy · 2008Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The bacterial lacZ gene encoding for beta-galactosidase (beta-gal) is a common reporter gene used in transgenic mice. Nonetheless, the absence of fluorigenic substrates usable in live animals greatly hampered the non-invasive follow-up of this reporter gene expression. We used far-red fluorescence for imaging beta-Gal expression in live cells in vitro or in vivo. The 9H-(1,3-dichloro-9,9-dimethylacridin- 2-one-7-yl) beta-D-galactopyranoside substrate was used to monitor beta-Gal expression as a reporter of tumor growth, or of the physiological levels of an endogenous gene or of gene transfer in lung. A quantitative evaluation of this method as well as a comparison of its sensitivity with Firefly Luciferase-based bioluminescence was also performed. In vivo measurements showed that 10(3) beta-Gal tumor cells located under the skin were detectable. In deeper organs like lung, as little as 5 ng of beta-Gal or Luciferase enzymes per mg of proteins were measured, confirming that both techniques reached similar sensibilities. Nonetheless, quantitative comparison of beta-Gal levels measured with far-red imaging or with a standardized enzymatic evaluation after killing revealed that the 2D-fluorescent reflectance imaging method is submitted to a color-dependent disparity of the organs and cannot supply quantitative measurements but that a simple correction can be applied.
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Registered trials
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