ArticleMolecular biology of the cell2007
Distinct sequence elements of cyclin B1 promote localization to chromatin, centrosomes, and kinetochores during mitosis.
Article in Molecular biology of the cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 86 citations in OpenAlex.
- Developmental regulation of kinetochore phosphorylation determines mitotic fidelity.bioRxiv : the preprint server for biology · 2026Article
- Anaphase onset requires CKS-1-mediated destruction of securin in meiosis I and cyclin B1 in meiosis II.The Journal of cell biology · 2026Article
- Countervailing effects of cyclin isoforms A and B during mitosis.bioRxiv : the preprint server for biology · 2026Article
- Pentagamavunone-1 targets excessive MYCN/NCYM expression mediated by mitotic arrest to suppress hepatocellular carcinoma proliferation.Cancer gene therapy · 2025Article
- A distinct phase of cyclin B (Cdc13) nuclear export at mitotic entry inOpen biology · 2025Article
- High-throughput investigation of cyclin docking interactions reveals the complexity of motif binding determinants.Nature communications · 2025Article
- Plasticity of mitotic cyclins in promoting the G2-M transition.The Journal of cell biology · 2025Article
- Dengue virus modulates critical cell cycle regulatory proteins in human megakaryocyte cells.Scientific reports · 2025Article
- Positively charged specificity site in cyclin B1 is essential for mitotic fidelity.Nature communications · 2025Article
- Spatial control of the APC/C ensures the rapid degradation of cyclin B1.The EMBO journal · 2024Article
- CDK activity at the centrosome regulates the cell cycle.Cell reports · 2024Article
- Centrosomes and associated proteins in pathogenesis and treatment of breast cancer.Frontiers in oncology · 2024Review
- A specific dispiropiperazine derivative that arrests cell cycle, induces apoptosis, necrosis and DNA damage.Scientific reports · 2023Article
- Nuclear-cytoplasmic compartmentalization of cyclin B1-Cdk1 promotes robust timing of mitotic events.Cell reports · 2022Article
- Small changes in phospho-occupancy at the kinetochore-microtubule interface drive mitotic fidelity.The Journal of cell biology · 2022Article
- Mad2 promotes Cyclin B2 recruitment to the kinetochore for guiding accurate mitotic checkpoint.EMBO reports · 2022Article
- B1-type cyclins control microtubule organization during cell division in Arabidopsis.EMBO reports · 2022Article
- Evolution of CDK1 Paralog Specializations in a Lineage With Fast Developing Planktonic Embryos.Frontiers in cell and developmental biology · 2021Article
- Rashomon at the kinetochore: Function(s) of the Mad1-cyclin B1 complex.The Journal of cell biology · 2020Article
- Cyclin B1 scaffolds MAD1 at the kinetochore corona to activate the mitotic checkpoint.The EMBO journal · 2020Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The mitotic cyclins promote cell division by binding and activating cyclin-dependent kinases (CDKs). Each cyclin has a unique pattern of subcellular localization that plays a vital role in regulating cell division. During mitosis, cyclin B1 is known to localize to centrosomes, microtubules, and chromatin. To determine the mechanisms of cyclin B1 localization in M phase, we imaged full-length and mutant versions of human cyclin B1-enhanced green fluorescent protein in live cells by using spinning disk confocal microscopy. In addition to centrosome, microtubule, and chromatin localization, we found that cyclin B1 also localizes to unattached kinetochores after nuclear envelope breakdown. Kinetochore recruitment of cyclin B1 required the kinetochore proteins Hec1 and Mad2, and it was stimulated by microtubule destabilization. Mutagenesis studies revealed that cyclin B1 is recruited to kinetochores through both CDK1-dependent and -independent mechanisms. In contrast, localization of cyclin B1 to chromatin and centrosomes is independent of CDK1 binding. The N-terminal domain of cyclin B1 is necessary and sufficient for chromatin association, whereas centrosome recruitment relies on sequences within the cyclin box. Our data support a role for cyclin B1 function at unattached kinetochores, and they demonstrate that separable and distinct sequence elements target cyclin B1 to kinetochores, chromatin, and centrosomes during mitosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.