Evidence map›Paper›PMID 17881548›Full record

ArticleJournal of clinical microbiology2007

Multilocus sequence typing compared to pulsed-field gel electrophoresis for molecular typing of Pseudomonas aeruginosa.

Jennifer K Johnson, Sonia M Arduino, O Colin Stine, Judith A Johnson, Anthony D Harris

Open access · greenAbstract readComparative Study
In one paragraph

Article in Journal of clinical microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.

0numbers the graph read from it
0cells of the map it votes in
49citing papers in PubMed
3.2field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

49 citing papers in PubMed, 107 citations in OpenAlex.

  1. The synergy ofThe Pan African medical journal · 2025
    Article
  2. Molecular Typing ofDiagnostics (Basel, Switzerland) · 2024
    Article
  3. Article
  4. Article
  5. Evaluation ofJAC-antimicrobial resistance · 2022
    Article
  6. Article
  7. Article
  8. Article
  9. Molecular Epidemiology of Carbapenemase-ProducingInfection and drug resistance · 2020
    Article
  10. Article
  11. Article
  12. Molecular characterization of clinical and environmentalSaudi journal of biological sciences · 2019
    Article
  13. Article
  14. Genotypic characterization ofInfection and drug resistance · 2019
    Article
  15. Review
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Jennifer K JohnsonDepartment of Pathology, University of Maryland School of Medicine, MSTF, Baltimore, MD 21201, USA. jkjohnson@som.umaryland.edu
Sonia M Arduino
O Colin Stine
Judith A Johnson
Anthony D Harris
University of Maryland, Baltimore · USVA Maryland Health Care System · US

Funding

Zonulin Antibodies in Intestinal Permeabilitiy in IDDMM01RR016500 · NCRR · UNIVERSITY OF MARYLAND BALTIMORE · PI OSWALD, LYNN M · 2002 to 2009
$20.2M
Epidemiology of imipenem-resistant P.aeruginosaR01AI060859 · NIAID · UNIVERSITY OF MARYLAND BALTIMORE · PI HARRIS, ANTHONY D · 2005 to 2013
$1.9M
Study design importance in analyzing emerging pathogensK23AI001752 · NIAID · UNIVERSITY OF MARYLAND BALTIMORE · PI HARRIS, ANTHONY D · 2001 to 2005
$662k
NCRR NIH HHS M01 RR016500NCRR NIH HHS M01 RR16500NIAID NIH HHS K23 AI001752NIAID NIH HHS K23 AI01752-01A1NIAID NIH HHS R01 AI060859NIAID NIH HHS R01 AI60859-01A1
6 · The paper itself

Abstract

For hospital epidemiologists, determining a system of typing that is discriminatory is essential for measuring the effectiveness of infection control measures. In situations in which the incidence of resistant Pseudomonas aeruginosa is increasing, the ability to discern whether it is due to patient-to-patient transmission versus an increase in patient endogenous strains is often made on the basis of molecular typing. The present study compared the discriminatory abilities of pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) for 90 P. aeruginosa isolates obtained from cultures of perirectal surveillance swabs from patients in an intensive care unit. PFGE identified 85 distinct types and 76 distinct groups when similarity cutoffs of 100% and 87%, respectively, were used. By comparison, MLST identified 60 sequence types that could be clustered into 11 clonal complexes and 32 singletons. By using the Simpson index of diversity (D), PFGE had a greater discriminatory ability than MLST for P. aeruginosa isolates (D values, 0.999 versus 0.975, respectively). Thus, while MLST was better for detecting genetic relatedness, we determined that PFGE was more discriminatory than MLST for determining genetic differences in P. aeruginosa.

Indexed as

Bacterial Typing TechniquesElectrophoresis, Gel, Pulsed-FieldHumansPseudomonas aeruginosaSequence Analysis, DNA

Identifiers

PMID17881548
PMCPMC2168506
OpenAlexW2104019857

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.