ArticleJournal of cellular biochemistry2008
Mouse spermatozoa contain a nuclease that is activated by pretreatment with EGTA and subsequent calcium incubation.
Article in Journal of cellular biochemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 39 citations in OpenAlex.
- Article
- An intracellular, non-oxidative factor activates in vitro chromatin fragmentation in pig sperm.Biological research · 2023Article
- Sperm degradation after vasectomy follows a sperm chromatin fragmentation-dependent mechanism causing DNA breaks in the toroid linker regions.Molecular human reproduction · 2022Article
- Functional Aspects of Sperm Chromatin Organization.Results and problems in cell differentiation · 2022Review
- Fatherhood and Sperm DNA Damage in Testicular Cancer Patients.Frontiers in endocrinology · 2018Review
- Luminal fluid of epididymis and vas deferens contributes to sperm chromatin fragmentation.Human reproduction (Oxford, England) · 2015Article
- Investigation on the Origin of Sperm DNA Fragmentation: Role of Apoptosis, Immaturity and Oxidative Stress.Molecular medicine (Cambridge, Mass.) · 2015Article
- Double-stranded DNA breaks hidden in the neutral Comet assay suggest a role of the sperm nuclear matrix in DNA integrity maintenance.Molecular human reproduction · 2014Article
- Isolation of sperm nuclei and nuclear matrices from the mouse, and other rodents.Methods in molecular biology (Clifton, N.J.) · 2013Article
- Preservation of mouse sperm by convective drying and storing in 3-O-methyl-D-glucose.PloS one · 2012Article
- Short-term storage of human spermatozoa in electrolyte-free medium without freezing maintains sperm chromatin integrity better than cryopreservation.Biology of reproduction · 2011Article
- Ca2+, Mg2+-dependent DNase involvement in apoptotic effects in spermatozoa of sea urchin Strongylocentrotus intermedius induced by two-headed sphingolipid rhizochalin.Marine biotechnology (New York, N.Y.) · 2011Article
- Poly(ADP-ribose) polymerases PARP1 and PARP2 modulate topoisomerase II beta (TOP2B) function during chromatin condensation in mouse spermiogenesis.Biology of reproduction · 2011Article
- Mammalian sperm chromatin as a model for chromatin function in DNA degradation and DNA replication.Systems biology in reproductive medicine · 2011Review
- Deficiency in mouse Y chromosome long arm gene complement is associated with sperm DNA damage.Genome biology · 2010Article
- A novel nuclease activity that is activated by Ca(2+) chelated to EGTA.Systems biology in reproductive medicine · 2009Article
- A model for the control of DNA integrity by the sperm nuclear matrix.Asian journal of andrologyReview
- Oxidative stress and male reproductive health.Asian journal of andrologyReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We demonstrated that mouse spermatozoa cleave their DNA into approximately 50 kb loop-sized fragments with topoisomerase IIB when treated with MnCl(2) and CaCl(2) in a process we term sperm chromatin fragmentation (SCF). SCF can be reversed by EDTA. A nuclease then further degrades the DNA in a process we term sperm DNA degradation (SDD). MnCl(2) alone could elicit this activity, but CaCl(2) had no effect. Here, we demonstrate the existence of a nuclease in the vas deferens that can be activated by ethylene glycol tetraacetic acid (EGTA) to digest the sperm DNA by SDD. Spermatozoa were extracted with salt and dithiothreitol to remove protamines and then incubated with EGTA. Next, the EGTA was removed and divalent cations were added. We found that Mn(2+), Ca(2+), or Zn(2+) could each activate SDD in spermatozoa but Mg(2+) could not. When the reaction was slowed by incubation on ice, EGTA pretreatment followed by incubation in Ca(2+) elicited the reversible fragmentation of sperm DNA evident in SCF. When the reactions were then incubated at 37 degrees C they progressed to the more complete degradation of DNA by SDD. EDTA could also be used to activate the nuclease, but required a higher concentration than EGTA. This EGTA-activatable nuclease activity was found in each fraction of the vas deferens plasma: in the spermatozoa, in the surrounding fluid, and in the insoluble components in the fluid. These results suggest that this sperm nuclease is regulated by a mechanism that is sensitive to EGTA, possibly by removing inhibition of a calcium binding protein.
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