ArticlePloS one2007
Connector inversion probe technology: a powerful one-primer multiplex DNA amplification system for numerous scientific applications.
Article in PloS one, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Dealing with Pseudogenes in Molecular Diagnostics in the Next Generation Sequencing Era.Methods in molecular biology (Clifton, N.J.) · 2021Review
- Quantitative assessment of LASSO probe assembly and long-read multiplexed cloning.BMC biotechnology · 2019Article
- High-Fidelity Nanopore Sequencing of Ultra-Short DNA Targets.Analytical chemistry · 2019Article
- Efficient in situ barcode sequencing using padlock probe-based BaristaSeq.Nucleic acids research · 2018Article
- Targeted next-generation sequencing for the detection of ciprofloxacin resistance markers using molecular inversion probes.Scientific reports · 2016Article
- Depletion of Abundant Sequences by Hybridization (DASH): using Cas9 to remove unwanted high-abundance species in sequencing libraries and molecular counting applications.Genome biology · 2016Article
- Molecular approaches to recognize relevant and emerging infectious diseases in animals.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Improved coverage and accuracy with strand-conserving sequence enrichment.Genome medicine · 2013Article
- Multiplex target capture with double-stranded DNA probes.Genome medicine · 2013Article
- Article
- Review of massively parallel DNA sequencing technologies.The HUGO journal · 2011Article
- Template tailoring: Accurate determination of heterozygous alleles using peptide nucleic acid and dideoxyNTP.Electrophoresis · 2010Article
- Targeted deep resequencing of the human cancer genome using next-generation technologies.Biotechnology & genetic engineering reviews · 2010Review
- Multiplex padlock targeted sequencing reveals human hypermutable CpG variations.Genome research · 2009Article
- Universal ligation-detection-reaction microarray applied for compost microbes.BMC microbiology · 2008Article
- Keeping up with the next generation: massively parallel sequencing in clinical diagnostics.The Journal of molecular diagnostics : JMD · 2008Review
- Nested Patch PCR enables highly multiplexed mutation discovery in candidate genes.Genome research · 2008Article
- A comprehensive assay for targeted multiplex amplification of human DNA sequences.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
We combined components of a previous assay referred to as Molecular Inversion Probe (MIP) with a complete gap filling strategy, creating a versatile powerful one-primer multiplex amplification system. As a proof-of-concept, this novel method, which employs a Connector Inversion Probe (CIPer), was tested as a genetic tool for pathogen diagnosis, typing, and antibiotic resistance screening with two distinct systems: i) a conserved sequence primer system for genotyping Human Papillomavirus (HPV), a cancer-associated viral agent and ii) screening for antibiotic resistance mutations in the bacterial pathogen Neisseria gonorrhoeae. We also discuss future applications and advances of the CIPer technology such as integration with digital amplification and next-generation sequencing methods. Furthermore, we introduce the concept of two-dimension informational barcodes, i.e. "multiplex multiplexing padlocks" (MMPs). For the readers' convenience, we also provide an on-line tutorial with user-interface software application CIP creator 1.0.1, for custom probe generation from virtually any new or established primer-pairs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.