ArticleProceedings of the National Academy of Sciences of the United States of America2007
Yeast screens show aromatic residues at the end of the sixth helix anchor transient receptor potential channel gate.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 48 citations in OpenAlex.
- Aquaporin ion conductance properties defined by membrane environment, protein structure, and cell physiology.Biophysical reviews · 2022Review
- Structure of the ancient TRPY1 channel from Saccharomyces cerevisiae reveals mechanisms of modulation by lipids and calcium.Structure (London, England : 1993) · 2022Article
- A Transient Receptor Potential-like Calcium Ion Channel in the Filamentous FungusJournal of fungi (Basel, Switzerland) · 2021Article
- Transient receptor potential channels: current perspectives on evolution, structure, function and nomenclature.Proceedings. Biological sciences · 2020Article
- Novel compounds that specifically bind and modulate MscL: insights into channel gating mechanisms.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Identification of Inhibitory CaiScience · 2019Article
- PI(3,5)PMolecular biology of the cell · 2018Article
- Scanning MscL Channels with Targeted Post-Translational Modifications for Functional Alterations.PloS one · 2015Article
- Differential regulation of staphylococcal virulence by the sensor kinase SaeS in response to neutrophil-derived stimuli.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Progressive degeneration of dopaminergic neurons through TRP channel-induced cell death.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Chimeras reveal a single lipid-interface residue that controls MscL channel kinetics as well as mechanosensitivity.Cell reports · 2013Article
- Sensing and responding to membrane tension: the bacterial MscL channel as a model system.Biophysical journal · 2012Review
- The core domain as the force sensor of the yeast mechanosensitive TRP channel.The Journal of general physiology · 2011Article
- Multiple modalities converge on a common gate to control K2P channel function.The EMBO journal · 2011Article
- The transient receptor potential family of ion channels.Genome biology · 2011Review
- Functional and structural studies of TRP channels heterologously expressed in budding yeast.Advances in experimental medicine and biology · 2011Review
- Forward genetic analysis reveals multiple gating mechanisms of TRPV4.The Journal of biological chemistry · 2010Article
- The tension-transmitting 'clutch' in the mechanosensitive channel MscS.Nature structural & molecular biology · 2010Article
- Functional and structural identification of amino acid residues of the P2X2 receptor channel critical for the voltage- and [ATP]-dependent gating.The Journal of physiology · 2009Article
- The use of yeast to understand TRP-channel mechanosensitivity.Pflugers Archiv : European journal of physiology · 2009Review
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Transient receptor potential (TRP) channels are first elements in sensing chemicals, heat, and force and are widespread among protists and fungi as well as animals. Despite their importance, the arrangement and roles of the amino acids that constitute the TRP channel gate are unknown. The yeast TRPY1 is activated in vivo by osmotically induced vacuolar membrane deformation and by cytoplasmic Ca(2+). After a random mutagenesis, we isolated TRPY1 mutants that responded more strongly to mild osmotic upshocks. One such gain-of-function mutant has a Y458H substitution at the C terminus of the predicted sixth transmembrane helix. Direct patch-clamp examination of vacuolar membranes showed that Y458H channels were already active with little stimulus and showed marked flickers between the open and intraburst closed states. They remained responsive to membrane stretch force and to Ca(2+), indicating primary defects in the gate region but not in the sensing of gating principles. None of the other 18 amino acid replacements engineered here showed normal channel kinetics except the two aromatic substitutions, Y458F and Y458W. The Y458 of TRPY1 has its aromatic counterpart in mammalian TRPM. Furthermore, conserved aromatics one alpha-helical turn downstream from this point are also found in animal TRPC, TRPN, TRPP, and TRPML, suggesting that gate anchoring with aromatics may be common among many TRP channels. The possible roles of aromatics at the end of the sixth transmembrane helix are discussed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.