ArticleProceedings of the National Academy of Sciences of the United States of America2007
Emergence of a fast-reacting ribozyme that is capable of undergoing continuous evolution.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Article
- In search of the RNA world on Mars.Geobiology · 2021Article
- Investigating the Evolution and Development of Biological Systems from the Perspective of Thermo-Kinetics and Systems Theory.Origins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life · 2020Review
- The developing toolkit of continuous directed evolution.Nature chemical biology · 2020Review
- In vivo continuous directed evolution.Current opinion in chemical biology · 2015Review
- Limits of neutral drift: lessons from the in vitro evolution of two ribozymes.Journal of molecular evolution · 2014Article
- Mechanisms of RNA catalysis.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2011Article
- Continuous evolution: protein evolution at warp speed.Nature chemical biology · 2011Article
- Deep sequencing analysis of mutations resulting from the incorporation of dNTP analogs.Nucleic acids research · 2010Article
- Closing the circle: replicating RNA with RNA.Cold Spring Harbor perspectives in biology · 2010Review
- Microfluidic landscapes for evolution.Current opinion in chemical biology · 2010Review
- The origins of cellular life.Cold Spring Harbor perspectives in biology · 2010Review
- Generation and development of RNA ligase ribozymes with modular architecture through "design and selection".Molecules (Basel, Switzerland) · 2010Review
- Microfluidic compartmentalized directed evolution.Chemistry & biology · 2010Article
- Computational generation and screening of RNA motifs in large nucleotide sequence pools.Nucleic acids research · 2010Article
- Evolutionary optimization of a modular ligase ribozyme: a small catalytic unit and a hairpin motif masking an element that could form an inactive structure.Nucleic acids research · 2010Article
- Quasispecies-like behavior observed in catalytic RNA populations evolving in a test tube.BMC evolutionary biology · 2010Article
- Rational optimization of the DSL ligase ribozyme with GNRA/receptor interacting modules.Archives of biochemistry and biophysics · 2009Article
- Niche partitioning in the coevolution of 2 distinct RNA enzymes.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Tailoring RNA modular units on a common scaffold: a modular ribozyme with a catalytic unit for beta-nicotinamide mononucleotide-activated RNA ligation.RNA (New York, N.Y.) · 2009Article
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2 authors.
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No grant is acknowledged in the PubMed record.
Abstract
It is possible to evolve RNA enzymes in a continuous manner by employing a simple serial-transfer procedure. This method was previously applied only to descendants of one unusually fast-reacting RNA enzyme with RNA ligase activity. The present study establishes a second continuously evolving RNA enzyme, also with RNA ligase activity, but with a completely independent evolutionary origin. Critical to achieving the fast catalytic rate necessary for continuous evolution, development of this enzyme entailed the addition and evolutionary maturation of a 35-nucleotide accessory domain and the application of highly stringent selection pressure, with reaction times as short as 15 ms. Once established, continuous evolution was carried out for 80 successive transfers, maintaining the population against an overall dilution of 10(207)-fold. The resulting RNA enzymes exhibited approximately 10(5)-fold improvement in catalytic efficiency, compared with the starting molecules, and became dependent on a structural feature of the substrate that previously conferred no selective advantage. This adaptation was eliminated by deleting the substrate feature and then carrying out 20 additional transfers of continuous evolution, which resulted in molecules with even greater catalytic activity. Now that two distinct species of continuously evolving enzymes have been established, it is possible to conduct molecular ecology experiments in which the two are made to compete for limited resources within a common environment.
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