ArticleIn vitro cellular & developmental biology. Animal
Differentiation potential of a basal epithelial cell line established from human bronchial explant.
Article in In vitro cellular & developmental biology. Animal. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 54 citations in OpenAlex.
- Beyond cysts - organization of epithelial networks in the murine thymus.Journal of cell science · 2025Article
- The Non-Antibacterial Effects of Azithromycin and Other Macrolides on the Bronchial Epithelial Barrier and Cellular Differentiation.International journal of molecular sciences · 2025Article
- Bile acid metabolites enhance expression of cathelicidin antimicrobial peptide in airway epithelium through activation of the TGR5-ERK1/2 pathway.Scientific reports · 2024Article
- In vitro modelling of bacterial pneumonia: a comparative analysis of widely applied complex cell culture models.FEMS microbiology reviews · 2024Review
- The effect of multiple outgrowths from bronchial tissue explants on progenitor/stem cell number in primary bronchial epithelial cell cultures from smokers and patients with COPD.Frontiers in medicine · 2023Article
- Imaging-guided bioreactor for de-epithelialization and long-term cultivation ofLab on a chip · 2022Article
- The Novel Inducer of Innate Immunity HO53 Stimulates Autophagy in Human Airway Epithelial Cells.Journal of innate immunity · 2022Article
- Establishment of human airway epithelial cells with doxycycline-inducible cell growth and fluorescence reporters.Cytotechnology · 2021Article
- Host and Pathogen Communication in the Respiratory Tract: Mechanisms and Models of a Complex Signaling Microenvironment.Frontiers in medicine · 2020Review
- Azithromycin induces epidermal differentiation and multivesicular bodies in airway epithelia.Respiratory research · 2019Article
- Novel aroylated phenylenediamine compounds enhance antimicrobial defense and maintain airway epithelial barrier integrity.Scientific reports · 2019Article
- Optimization of Normal Human Bronchial Epithelial (NHBE) Cell 3D Cultures for in vitro Lung Model Studies.Scientific reports · 2019Article
- Bordetella pertussis Adenylate Cyclase Toxin Disrupts Functional Integrity of Bronchial Epithelial Layers.Infection and immunity · 2018Article
- Epithelial Plasticity During Human Breast Morphogenesis and Cancer Progression.Journal of mammary gland biology and neoplasia · 2016Review
- Chlamydia trachomatis Infection of Endocervical Epithelial Cells Enhances Early HIV Transmission Events.PloS one · 2016Article
- Basal cells of the human airways acquire mesenchymal traits in idiopathic pulmonary fibrosis and in culture.Laboratory investigation; a journal of technical methods and pathology · 2015Article
- Curcumin, bisdemethoxycurcumin and dimethoxycurcumin complexed with cyclodextrins have structure specific effect on the paracellular integrity of lung epitheliaBiochemistry and biophysics reports · 2015Article
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- Conditions associated with the cystic fibrosis defect promote chronic Pseudomonas aeruginosa infection.American journal of respiratory and critical care medicine · 2014Article
- Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Due to the cellular complexity of the airway epithelium, it is important to carefully define bronchial cell lines that capture the phenotypic traits of a particular cell type. We describe the characterization of a human bronchial epithelial cell line, VA10. It was established by transfection of primary bronchial epithelial cells with retroviral constructs containing the E6 and E7 oncogenes from HPV16. The cell line has been cultured for over 2 yr, a total of 60 passages. Although prolonged culture resulted in increased chromosomal instability, no major phenotypic drift in marker expression was observed. The cells expressed cytokeratins 5, 13, 14, and 17 suggesting a basal-like phenotype. This was further supported by the expression of alpha6beta4 integrins and the basal cell-associated transcription factor p63. The VA10 cell line generated high transepithelial electrical resistance in suspended and air-liquid interface culture, indicating functionally active tight junction (TJ) complexes. Immunocytochemistry showed the typical reticular structures of occludin and TJ-associated F-actin. VA10 produced pseudostratified layer in air-liquid interface culture with expression of p63 restricted to the basal layer. Furthermore, VA10 produced round colonies when cultured in laminin-rich reconstituted basement membrane, and immunostaining of claudin-1 and the basolateral marker beta4 integrin revealed colonies that generated polarization as expected in vivo. These data indicate that VA10 epithelia have the potential to model the bronchial epithelium in vivo and may be useful to study epithelial regeneration and repair and the effect of chemicals and potential drug candidates on TJ molecules in airway epithelia.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.