ArticleMolecular and cellular biology2007
Vitamin K induces osteoblast differentiation through pregnane X receptor-mediated transcriptional control of the Msx2 gene.
Article in Molecular and cellular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 36 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
36 citing papers in PubMed, 79 citations in OpenAlex.
- Current knowledge of bone-derived factor osteocalcin: its role in the management and treatment of diabetes mellitus, osteoporosis, osteopetrosis and inflammatory joint diseases.Journal of molecular medicine (Berlin, Germany) · 2024Review
- Review
- The role of pregnane X receptor (PXR) in substance metabolism.Frontiers in endocrinology · 2022Review
- Review
- High Pregnane X Receptor (PXR) Expression Is Correlated with Poor Prognosis in Invasive Breast Carcinoma.Diagnostics (Basel, Switzerland) · 2021Article
- Vitamin K-Dependent Proteins in Skeletal Development and Disease.International journal of molecular sciences · 2021Review
- Menaquinone-4 Suppresses Lipopolysaccharide-Induced Inflammation in MG6 Mouse Microglia-Derived Cells by Inhibiting the NF-κB Signaling Pathway.International journal of molecular sciences · 2019Article
- Vitamin K and Bone Metabolism: A Review of the Latest Evidence in Preclinical Studies.BioMed research international · 2018Review
- Combined treatment of vitamin K and teriparatide on bone metabolism and biomechanics in rats with osteoporosis.Experimental and therapeutic medicine · 2018Article
- Protective effect of VK2 on glucocorticoid-treated MC3T3-E1 cells.International journal of molecular medicine · 2017Article
- Pregnane X Receptor and Cancer: Context-Specificity is Key.Nuclear receptor research · 2016Article
- Effects of the combination of vitamin K and teriparatide on the bone metabolism in ovariectomized rats.Biomedical reports · 2015Article
- Review
- Dietary supplementation with vitamin k affects transcriptome and proteome of Senegalese sole, improving larval performance and quality.Marine biotechnology (New York, N.Y.) · 2014Article
- Post-translational and post-transcriptional modifications of pregnane X receptor (PXR) in regulation of the cytochrome P450 superfamily.Current drug metabolism · 2013Review
- Increased invasiveness of osteosarcoma mesenchymal stem cells induced by bone-morphogenetic protein-2.In vitro cellular & developmental biology. Animal · 2013Article
- UBIAD1-mediated vitamin K2 synthesis is required for vascular endothelial cell survival and development.Development (Cambridge, England) · 2013Article
- Pregnane xenobiotic receptor in cancer pathogenesis and therapeutic response.Cancer letters · 2013Review
- Comparison of the characteristics of mesenchymal stem cells obtained from prostate tumors and from bone marrow cultured in conditioned medium.Experimental and therapeutic medicine · 2012Article
- Is macroporosity absolutely required for preliminary in vitro bone biomaterial study? A comparison between porous materials and flat materials.Journal of functional biomaterials · 2011Article
Corrections and comments
- Retraction · 2014-03-01Concerns/Issues about Data · Concerns/Issues about Image · Ethical Violations by Author · Manipulation of Images · Objections by Author(s) · Unreliable Results and/or Conclusions · · The retraction notes that 'M. Igarashi and H. Kitagawa did not agree to this retraction.'; There is an earlier Expression of Concern for this article.;
- Expression of concern · 2013-06-01Concerns/Issues about Data · Concerns/Issues about Image · Investigation by Company/Institution · Manipulation of Images ·
- Expression of concern
- Retracted
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Vitamin K is a fat-soluble vitamin that serves as a coenzyme for vitamin K-dependent carboxylase. Besides its canonical action, vitamin K binds to the steroid and xenobiotic receptor (SXR)/pregnane X receptor (PXR) and modulates gene transcription. To determine if the osteoprotective action of vitamin K is the result of the PXR/SXR pathway, we screened by two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis the PXR/SXR target genes in an osteoblastic cell line (MC3T3-E1) treated with a vitamin K2 (menaquinone 4 [MK4]). Osteoblastic differentiation of MC3T3-E1 cells was induced by MK4. Msx2, an osteoblastogenic transcription factor, was identified as an MK4-induced gene. Functional analysis of the Msx2 gene promoter mapped a vitamin K-responsive element (PXR-responsive element [PXRE]) that was directly bound by a PXR/retinoid X receptor alpha heterodimer. In a chromatin immunoprecipitation analysis, PXR was recruited together with a coactivator, p300, to the PXRE in the Msx2 promoter. MK4-bound PXR cooperated with estrogen-bound estrogen receptor alpha to control transcription at the Msx2 promoter. Knockdown of either PXR or Msx2 attenuated the effect of MK4 on osteoblastic differentiation. Thus, the present study suggests that Msx2 is a target gene for PXR activated by vitamin K and suggests that the osteoprotective action of MK4 in the human mediates, at least in part, a genomic pathway of vitamin K signaling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.