ArticleMolecular and cellular biology2007
In vivo analysis of developmentally and evolutionarily dynamic protein-DNA interactions regulating transcription of the Pgk2 gene during mammalian spermatogenesis.
Article in Molecular and cellular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 46 citations in OpenAlex.
- Article
- Article
- Proteomic biomarkers in seminal plasma as predictors of reproductive potential in azoospermic men.Frontiers in endocrinology · 2024Article
- Novel Aspects of cAMP-Response Element Modulator (CREM) Role in Spermatogenesis and Male Fertility.International journal of molecular sciences · 2023Review
- Non-Invasive Diagnostics of Male Spermatogenesis from Seminal Plasma: Seminal Proteins.Diagnostics (Basel, Switzerland) · 2023Review
- Identifying the Potential Roles of PBX4 in Human Cancers Based on Integrative Analysis.Biomolecules · 2022Article
- Combined Analysis of Gut Microbiota and Plasma Metabolites Reveals the Effect of Red-Fleshed Apple Anthocyanin Extract on Dysfunction of Mice Reproductive System Induced by Busulfan.Frontiers in nutrition · 2021Article
- Article
- Response to: X-linked miR-506 family miRNAs promote FMRP expression in mouse spermatogonia.EMBO reports · 2020Article
- Germ cell-intrinsic requirement for the homeodomain transcription factor PKnox1/Prep1 in adult spermatogenesis.PloS one · 2018Article
- Article
- Dephosphorylation of Tak1 at Ser412 greatly contributes to the spermatocyte-specific testis toxicity induced by (5R)-5-hydroxytriptolide in C57BL/6 mice.Toxicology research · 2016Article
- Sequence-specific promoter elements regulate temporal-specific changes in chromatin required for testis-specific activation of the Pgk2 gene.Reproduction (Cambridge, England) · 2013Article
- A-MYB (MYBL1) stimulates murine testis-specific Ldhc expression via the cAMP-responsive element (CRE) site.Biology of reproduction · 2012Article
- MYBBP1A: a new Ipr1's binding protein in mice.Molecular biology reports · 2010Article
- Microarray-based analysis of cell-cycle gene expression during spermatogenesis in the mouse.Biology of reproduction · 2010Article
- Phosphoglycerate kinase 2 (PGK2) is essential for sperm function and male fertility in mice.Biology of reproduction · 2010Article
- Activation and repression domains within the promoter of the rat cathepsin L gene orchestrate sertoli cell-specific and stage-specific gene transcription in transgenic mice.Biology of reproduction · 2009Article
- Identification and characterization of a novel testis-specific gene CKT2, which encodes a substrate for protein kinase CK2.Nucleic acids research · 2009Article
- RNA-based gene duplication: mechanistic and evolutionary insights.Nature reviews. Genetics · 2009Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Transcription of the testis-specific Pgk2 gene is selectively activated in primary spermatocytes to provide a source of phosphoglycerate kinase that is critical to normal motility and fertility of mammalian spermatozoa. We examined dynamic changes in protein-DNA interactions at the Pgk2 gene promoter during murine spermatogenesis in vivo by performing genomic footprinting and chromatin immunoprecipitation assays with enriched populations of murine spermatogenic cells at stages prior to, during, and following transcription of this gene. We found that genes encoding the testis-specific homeodomain factor PBX4 and its coactivator, PREP1, are expressed in patterns that mirror expression of the Pgk2 gene and that these factors become bound to the Pgk2 enhancer in cells in which this gene is actively expressed. We therefore suggest that these factors, along with CREM and SP3, direct stage- and cell type-specific transcription of the Pgk2 gene during spermatogenesis. We propose that binding of PBX4, plus its coactivator PREP1, is a rate-limiting step leading to the initiation of tissue-specific transcription of the Pgk2 gene. This study provides insight into the developmentally dynamic establishment of tissue-specific protein-DNA interactions in vivo. It also allows us to speculate about the events that led to tissue-specific regulation of the Pgk2 gene during mammalian evolution.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.