ArticleBMC genomics2007
Analysis of the immune-inducible transcriptome from microbial stress resistant, rat-tailed maggots of the drone fly Eristalis tenax.
Article in BMC genomics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 56 citations in OpenAlex.
- Laboratory risk assessment of Beauveria bassiana AAD16 on two species of ladybird beetle.PloS one · 2025Article
- Insect Antimicrobial Peptides as Guardians of Immunity and Beyond: A Review.International journal of molecular sciences · 2024Review
- Antimicrobial Peptides from Rat-Tailed Maggots of the Drone FlyMicroorganisms · 2020Article
- A world review of reported myiases caused by flower flies (Diptera: Syrphidae), including the first case of human myiasis from Palpada scutellaris (Fabricius, 1805).Parasitology research · 2020Review
- Rearing and Long-Term Maintenance of Eristalis tenax Hoverflies for Research Studies.Journal of visualized experiments : JoVE · 2018Article
- Rapid Expansion of Immune-Related Gene Families in the House Fly, Musca domestica.Molecular biology and evolution · 2017Article
- Seasonal phenotype-specific transcriptional reprogramming during metamorphosis in the European map butterflyEcology and evolution · 2016Article
- Perspectives on the evolutionary ecology of arthropod antimicrobial peptides.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2016Review
- Diversity, evolution and medical applications of insect antimicrobial peptides.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2016Review
- Sex, offspring and carcass determine antimicrobial peptide expression in the burying beetle.Scientific reports · 2016Article
- Antimicrobial peptides expressed in medicinal maggots of the blow fly Lucilia sericata show combinatorial activity against bacteria.Antimicrobial agents and chemotherapy · 2015Article
- Article
- Changes in the transcriptome of the malaria parasite Plasmodium falciparum during the initial phase of transmission from the human to the mosquito.BMC genomics · 2013Article
- Article
- Next generation sequencing based transcriptome analysis of septic-injury responsive genes in the beetle Tribolium castaneum.PloS one · 2013Article
- A comprehensive transcriptome and immune-gene repertoire of the lepidopteran model host Galleria mellonella.BMC genomics · 2011Article
- Immunity and other defenses in pea aphids, Acyrthosiphon pisum.Genome biology · 2010Article
- Comparative analysis of septic injury-inducible genes in phylogenetically distant model organisms of regeneration and stem cell research, the planarian Schmidtea mediterranea and the cnidarian Hydra vulgaris.Frontiers in zoology · 2008Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe saprophagous and coprophagous maggots of the drone fly Eristalis tenax (Insecta, Diptera) have evolved the unique ability to survive in aquatic habitats with extreme microbial stress such as drains, sewage pools, and farmyard liquid manure storage pits. Therefore, they represent suitable models for the investigation of trade-offs between the benefits resulting from colonization of habitats lacking predators, parasitoids, or competitors and the investment in immunity against microbial stress. In this study, we screened for genes in E. tenax that are induced upon septic injury. Suppression subtractive hybridization was performed to selectively amplify and identify cDNAs that are differentially expressed in response to injected crude bacterial endotoxin (LPS).
resultsUntreated E. tenax maggots exhibit significant antibacterial activity in the hemolymph which strongly increases upon challenge with LPS. In order to identify effector molecules contributing to this microbial defense we constructed a subtractive cDNA library using RNA samples from untreated and LPS injected maggots. Analysis of 288 cDNAs revealed induced expression of 117 cDNAs corresponding to 30 novel gene clusters in E. tenax. Among these immune-inducible transcripts we found homologues of known genes from other Diptera such as Drosophila and Anopheles that mediate pathogen recognition (e.g. peptidoglycan recognition protein) or immune-related signaling (e.g. relish). As predicted, we determined a high diversity of novel putative antimicrobial peptides including one E. tenax defensin.
conclusionWe identified 30 novel genes of E. tenax that were induced in response to septic injury including novel putative antimicrobial peptides. Further analysis of these immune-related effector molecules from Eristalis may help to elucidate the interdependency of ecological adaptation and molecular evolution of the innate immunity in Diptera.
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