ArticleChromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology2007
Inhibition of transcription at radiation-induced nuclear foci of phosphorylated histone H2AX in mammalian cells.
Article in Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 40 citations in OpenAlex.
- Checkpoint Responses to DNA Double-Strand Breaks.Annual review of biochemistry · 2020Review
- De novo phosphorylation of H2AX by WSTF regulates transcription-coupled homologous recombination repair.Nucleic acids research · 2019Article
- Jumonji Inhibitors Overcome Radioresistance in Cancer through Changes in H3K4 Methylation at Double-Strand Breaks.Cell reports · 2018Article
- MicroRNA-3196 is inhibited by H2AX phosphorylation and attenuates lung cancer cell apoptosis by downregulating PUMA.Oncotarget · 2016Article
- Chromosome territory relocation during DNA repair requires nuclear myosin 1 recruitment to chromatin mediated by ϒ-H2AX signaling.Nucleic acids research · 2016Article
- DNA double-strand break repair is impaired in presenescent Syrian hamster fibroblasts.BMC molecular biology · 2015Article
- γ-H2AX Kinetic Profile in Mouse Lymphocytes Exposed to the Internal Emitters Cesium-137 and Strontium-90.PloS one · 2015Article
- Blurring the line between the DNA damage response and transcription: the importance of chromatin dynamics.Experimental cell research · 2014Review
- Hairpin-end conformation of adeno-associated virus genome determines interactions with DNA-repair pathways.Gene therapy · 2013Article
- SPO11-independent DNA repair foci and their role in meiotic silencing.PLoS genetics · 2013Article
- The dynamics of DNA damage repair and transcription.Methods in molecular biology (Clifton, N.J.) · 2013Article
- uPAR and cathepsin B inhibition enhanced radiation-induced apoptosis in gliomainitiating cells.Neuro-oncology · 2012Article
- Radiation-induced alterations in histone modification patterns and their potential impact on short-term radiation effects.Frontiers in oncology · 2012Article
- Cohesin protects genes against γH2AX Induced by DNA double-strand breaks.PLoS genetics · 2012Article
- Double-strand break-induced transcriptional silencing is associated with loss of tri-methylation at H3K4.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2011Article
- Histone tails: Directing the chromatin response to DNA damage.FEBS letters · 2011Review
- H2AX phosphorylation at the sites of DNA double-strand breaks in cultivated mammalian cells and tissues.Clinical epigenetics · 2011Article
- Differential effects of DNA double-strand break repair pathways on single-strand and self-complementary adeno-associated virus vector genomes.Journal of virology · 2010Article
- High-resolution profiling of gammaH2AX around DNA double strand breaks in the mammalian genome.The EMBO journal · 2010Article
- Gamma-H2AX in recognition and signaling of DNA double-strand breaks in the context of chromatin.Nucleic acids research · 2008Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Double-strand DNA breaks (DSBs) induced by ionizing radiation can be visualized in human cells using antibodies against Ser-139 phosphorylated histone H2AX (gamma-H2AX). Large gamma-H2AX foci are seen in the nucleus fixed 1 hour after irradiation and their number corresponds to the number of DSBs, allowing analysis of these genome lesions after low doses. We estimated whether transcription is affected in chromatin domains containing gamma-H2AX by following in vivo incorporation of 5-bromouridine triphosphate (BrUTP) loaded by cell scratching (run-on assay). We found that BrUTP incorporation is strongly suppressed at gamma-H2AX foci, suggesting that H2AX phosphorylation inhibits transcription. This is not caused by preferential association of gamma-H2AX foci with constitutive or facultative heterochromatin, which was visualized in irradiated cells using antibodies against histone H3 trimethylated at lysine-9 (H3-K9m3) or histone H3 trimethylated at lysine-27 (H3-K27m3). Apparently, formation of gamma-H2AX induces changes of chromatin that inhibit assembly of transcription complexes without heterochromatin formation. Inhibition of transcription by phosphorylation of histone H2AX can decrease chromatin movement at DSBs and frequency of misjoining of DNA ends.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.