ArticleEukaryotic cell2007
Small trypanosome RNA-binding proteins TbUBP1 and TbUBP2 influence expression of F-box protein mRNAs in bloodstream trypanosomes.
Article in Eukaryotic cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Transcriptomic analysis of N-terminal mutated Trypanosoma cruzi UBP1 knockdown underlines the importance of this RNA-binding protein in parasite development.PLoS neglected tropical diseases · 2024Article
- Chemical Inhibition of Bromodomain Proteins in Insect-Stage African Trypanosomes Perturbs Silencing of the Variant Surface Glycoprotein Repertoire and Results in Widespread Changes in the Transcriptome.Microbiology spectrum · 2023Article
- TheRNA (New York, N.Y.) · 2022Article
- Article
- Regulation of gene expression in trypanosomatids: living with polycistronic transcription.Open biology · 2019Article
- Expression of the RNA-binding protein RBP10 promotes the bloodstream-form differentiation state in Trypanosoma brucei.PLoS pathogens · 2017Article
- Untranslated regions of mRNA and their role in regulation of gene expression in protozoan parasites.Journal of biosciences · 2017Review
- Comparative genomics of drug resistance in Trypanosoma brucei rhodesiense.Cellular and molecular life sciences : CMLS · 2016Article
- Purification of Messenger Ribonucleoprotein Particles via a Tagged Nascent Polypeptide.PloS one · 2016Article
- Depletion of the Trypanosome Pumilio domain protein PUF2 or of some other essential proteins causes transcriptome changes related to coding region length.Eukaryotic cell · 2014Article
- The emerging role of RNA-binding proteins in the life cycle of Trypanosoma brucei.Cellular microbiology · 2014Review
- Trypanosome MKT1 and the RNA-binding protein ZC3H11: interactions and potential roles in post-transcriptional regulatory networks.Nucleic acids research · 2014Article
- Two splicing factors carrying serine-arginine motifs, TSR1 and TSR1IP, regulate splicing, mRNA stability, and rRNA processing in Trypanosoma brucei.RNA biology · 2014Article
- Proteins associated with SF3a60 in T. brucei.PloS one · 2014Article
- Basal splicing factors regulate the stability of mature mRNAs in trypanosomes.The Journal of biological chemistry · 2013Article
- The regulation of trypanosome gene expression by RNA-binding proteins.PLoS pathogens · 2013Review
- The trypanosome Pumilio domain protein PUF5.PloS one · 2013Article
- Trans-acting proteins regulating mRNA maturation, stability and translation in trypanosomatids.Trends in parasitology · 2011Review
- An RNA recognition motif mediates the nucleocytoplasmic transport of a trypanosome RNA-binding protein.The Journal of biological chemistry · 2009Article
- Widespread variation in transcript abundance within and across developmental stages of Trypanosoma brucei.BMC genomics · 2009Article
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13 authors.
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Abstract
In the African trypanosome Trypanosoma brucei nearly all control of gene expression is posttranscriptional; sequences in the 3'-untranslated regions of mRNAs determine the steady-state mRNA levels by regulation of RNA turnover. Here we investigate the roles of two related proteins, TbUBP1 and TbUBP2, containing a single RNA recognition motif, in trypanosome gene expression. TbUBP1 and TbUBP2 are in the cytoplasm and nucleus, comprise ca. 0.1% of the total protein, and are not associated with polysomes or RNA degradation enzymes. Overexpression of TbUBP2 upregulated the levels of several mRNAs potentially involved in cell division, including the CFB1 mRNA, which encodes a protein with a cyclin F-box domain. CFB1 regulation was mediated by the 3'-untranslated region and involved stabilization of the mRNA. Depletion of TbUBP2 and TbUBP1 inhibited growth and downregulated expression of the cyclin F box protein gene CFB2; trans splicing was unaffected. The results of pull-down assays indicated that all tested mRNAs were bound to TbUBP2 or TbUBP1, with some preference for CFB1. We suggest that TbUBP1 and TbUBP2 may be relatively nonspecific RNA-binding proteins and that specific effects of overexpression or depletion could depend on competition between various different proteins for RNA binding.
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