ArticleEukaryotic cell2007
Supramolecular organization of the respiratory chain in Neurospora crassa mitochondria.
Article in Eukaryotic cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Plasmodium falciparum stomatin-like protein forms a putative complex with a metalloprotease in distinct mitochondrial loci.PLoS pathogens · 2026Article
- Growth conditions shape the proteome and diversity ofCell surface (Amsterdam, Netherlands) · 2025Article
- Analysis of the Respiratory Activity in the Antarctic YeastMicroorganisms · 2024Article
- Regulation and functional role of the electron transport chain supercomplexes.Biochemical Society transactions · 2021Review
- The SPFH Protein Superfamily in Fungi: Impact on Mitochondrial Function and Implications in Virulence.Microorganisms · 2021Review
- Carbon and Nitrogen Sources Have No Impact on the Organization and Composition ofJournal of fungi (Basel, Switzerland) · 2021Article
- Mitochondrial respiratory supercomplexes in mammalian cells: structural versus functional role.Journal of molecular medicine (Berlin, Germany) · 2021Review
- Contribution of Complex I NADH Dehydrogenase to Respiratory Energy Coupling in Glucose-Grown Cultures ofApplied and environmental microbiology · 2020Article
- Research journey of respirasome.Protein & cell · 2020Review
- Increased reactive oxygen species production and maintenance of membrane potential in VDAC-less Neurospora crassa mitochondria.Journal of bioenergetics and biomembranes · 2019Article
- Cryo-EM structure ofIUCrJ · 2019Article
- Stay Fit, Stay Young: Mitochondria in Movement: The Role of Exercise in the New Mitochondrial Paradigm.Oxidative medicine and cellular longevity · 2019Review
- CytochromeComputational and structural biotechnology journal · 2019Review
- Respiratory chain supercomplexes: Structures, function and biogenesis.Seminars in cell & developmental biology · 2018Review
- Article
- Mitochondrial signaling in the vascular endothelium: beyond reactive oxygen species.Basic research in cardiology · 2016Review
- Protein Network of the Pseudomonas aeruginosa Denitrification Apparatus.Journal of bacteriology · 2016Article
- A Periplasmic Complex of the Nitrite Reductase NirS, the Chaperone DnaK, and the Flagellum Protein FliC Is Essential for Flagellum Assembly and Motility in Pseudomonas aeruginosa.Journal of bacteriology · 2015Article
- Mitochondrial type II NAD(P)H dehydrogenases in fungal cell death.Microbial cell (Graz, Austria) · 2015Review
- Respiratory supercomplexes: plasticity and implications.Frontiers in bioscience (Landmark edition) · 2015Review
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4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The existence of specific respiratory supercomplexes in mitochondria of most organisms has gained much momentum. However, its functional significance is still poorly understood. The availability of many deletion mutants in complex I (NADH:ubiquinone oxidoreductase) of Neurospora crassa, distinctly affected in the assembly process, offers unique opportunities to analyze the biogenesis of respiratory supercomplexes. Herein, we describe the role of complex I in assembly of respiratory complexes and supercomplexes as suggested by blue and colorless native polyacrylamide gel electrophoresis and mass spectrometry analyses of mildly solubilized mitochondria from the wild type and eight deletion mutants. As an important refinement of the fungal respirasome model, we found that the standard respiratory chain of N. crassa comprises putative complex I dimers in addition to I-III-IV and III-IV supercomplexes. Three Neurospora mutants able to assemble a complete complex I, lacking only the disrupted subunit, have respiratory supercomplexes, in particular I-III-IV supercomplexes and complex I dimers, like the wild-type strain. Furthermore, we were able to detect the I-III-IV supercomplexes in the nuo51 mutant with no overall enzymatic activity, representing the first example of inactive respirasomes. In addition, III-IV supercomplexes were also present in strains lacking an assembled complex I, namely, in four membrane arm subunit mutants as well as in the peripheral arm nuo30.4 mutant. In membrane arm mutants, high-molecular-mass species of the 30.4-kDa peripheral arm subunit comigrating with III-IV supercomplexes and/or the prohibitin complex were detected. The data presented herein suggest that the biogenesis of complex I is linked with its assembly into supercomplexes.
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