ArticleApplied and environmental microbiology2007
Sensitive and specific molecular detection of Burkholderia pseudomallei, the causative agent of melioidosis, in the soil of tropical northern Australia.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers, 1 of them a synthesis that pooled it.
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Who cites it
54 citing papers in PubMed, 1 synthesis or guideline pooled it, 80 citations in OpenAlex.
- Systematic review and consensus guidelines for environmental sampling of Burkholderia pseudomallei.PLoS neglected tropical diseases · 2013Pooled it
- Evaluation of culture- and PCR-based methods for detecting Burkholderia pseudomallei in soil samples in Thailand.PLoS neglected tropical diseases · 2026Article
- Geographical distribution of Burkholderia pseudomallei in Taiwanese croplands and the influence of bacterial community interactions on its incubation viability.PLoS neglected tropical diseases · 2025Article
- Spatial and Temporal Analyses of Melioidosis in Kaohsiung, Taiwan.The American journal of tropical medicine and hygiene · 2025Article
- Melioidosis in goats at a single Australian farm was caused by multiple diverse lineages of Burkholderia pseudomallei present in soil.PLoS neglected tropical diseases · 2024Article
- Exploring the Relationship between Melioidosis Morbidity Rate and Local Environmental Indicators Using Remotely Sensed Data.International journal of environmental research and public health · 2024Article
- Burkholderia pseudomallei Bacteria in Ornamental Fish Tanks, Vientiane, Laos, 2023.Emerging infectious diseases · 2024Article
- Rational design of an acidic erythritol (ACER) medium for the enhanced isolation of the environmental pathogenFrontiers in microbiology · 2023Article
- Environmental Factors Associated With Soil Prevalence of the Melioidosis PathogenFrontiers in microbiology · 2022Article
- Low risk of acquiring melioidosis from the environment in the continental United States.PloS one · 2022Article
- No hints for abundance of Bacillus anthracis and Burkholderia pseudomallei in 100 environmental samples from Cameroon.European journal of microbiology & immunology · 2021Article
- Using Land Runoff to Survey the Distribution and Genetic Diversity ofApplied and environmental microbiology · 2021Article
- Detection and differentiation of Burkholderia species with pathogenic potential in environmental soil samples.PloS one · 2021Article
- Whole-genome sequencing of Burkholderia pseudomallei from an urban melioidosis hot spot reveals a fine-scale population structure and localised spatial clustering in the environment.Scientific reports · 2020Article
- Human Melioidosis.Clinical microbiology reviews · 2020Review
- Burkholderia pseudomallei, the causative agent of melioidosis, is rare but ecologically established and widely dispersed in the environment in Puerto Rico.PLoS neglected tropical diseases · 2019Article
- A cluster of melioidosis infections in hatchling saltwater crocodiles (Microbial genomics · 2019Article
- A Study ofThe American journal of tropical medicine and hygiene · 2019Article
- Climatic drivers of melioidosis in Laos and Cambodia: a 16-year case series analysis.The Lancet. Planetary health · 2018Article
- Melioidosis in the Western Indian Ocean and the Importance of Improving Diagnosis, Surveillance, and Molecular Typing.Tropical medicine and infectious disease · 2018Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Burkholderia pseudomallei, the cause of the severe disease melioidosis in humans and animals, is a gram-negative saprophyte living in soil and water of areas of endemicity such as tropical northern Australia and Southeast Asia. Infection occurs mainly by contact with wet contaminated soil. The environmental distribution of B. pseudomallei in northern Australia is still unclear. We developed and evaluated a direct soil B. pseudomallei DNA detection method based on the recently published real-time PCR targeting the B. pseudomallei type III secretion system. The method was evaluated by inoculating different soil types with B. pseudomallei dilution series and by comparing B. pseudomallei detection rate with culture-based detection rate for 104 randomly collected soil samples from the Darwin rural area in northern Australia. We found that direct soil B. pseudomallei DNA detection not only was substantially faster than culture but also proved to be more sensitive with no evident false-positive results. This assay provides a new tool to detect B. pseudomallei in soil samples in a fast and highly sensitive and specific manner and is applicable for large-scale B. pseudomallei environmental screening studies or in outbreak situations. Furthermore, analysis of the 104 collected soil samples revealed a significant association between B. pseudomallei-positive sites and the presence of animals at these locations and also with moist, reddish brown-to-reddish gray soils.
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