ArticleJournal of bacteriology2007
Iteron-binding ORF157 and FtsZ-like ORF156 proteins encoded by pBtoxis play a role in its replication in Bacillus thuringiensis subsp. israelensis.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 29 citations in OpenAlex.
- Catching a Walker in the Act-DNA Partitioning by ParA Family of Proteins.Frontiers in microbiology · 2022Review
- Completed Genomic Sequence ofG3 (Bethesda, Md.) · 2020Article
- Role of plasmid plasticity and mobile genetic elements in the entomopathogen Bacillus thuringiensis serovar israelensis.FEMS microbiology reviews · 2018Article
- The TubR-centromere complex adopts a double-ring segrosome structure in Type III partition systems.Nucleic acids research · 2018Article
- Genetic Environment of cry1 Genes Indicates Their Common Origin.Genome biology and evolution · 2017Article
- Reconstitution of a prokaryotic minus end-tracking system using TubRC centromeric complexes and tubulin-like protein TubZ filaments.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- A novel transcriptional activator, tubX, is required for the stability of Bacillus sphaericus mosquitocidal plasmid pBsph.Journal of bacteriology · 2014Article
- Superstructure of the centromeric complex of TubZRC plasmid partitioning systems.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Filament formation of the FtsZ/tubulin-like protein TubZ from the Bacillus cereus pXO1 plasmid.The Journal of biological chemistry · 2012Article
- Centromere binding and evolution of chromosomal partition systems in the Burkholderiales.Journal of bacteriology · 2012Article
- Tubulin homolog TubZ in a phage-encoded partition system.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- A 54-kilodalton protein encoded by pBtoxis is required for parasporal body structural integrity in Bacillus thuringiensis subsp. israelensis.Journal of bacteriology · 2012Article
- Bacterial plasmid partition machinery: a minimalist approach to survival.Current opinion in structural biology · 2012Review
- Crystal structure and centromere binding of the plasmid segregation protein ParB from pCXC100.Nucleic acids research · 2011Article
- Filament structure of bacterial tubulin homologue TubZ.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- One-way ticket to the cell pole: plasmid transport by the prokaryotic tubulin homolog TubZ.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Plasmid protein TubR uses a distinct mode of HTH-DNA binding and recruits the prokaryotic tubulin homolog TubZ to effect DNA partition.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Structure and filament dynamics of the pSK41 actin-like ParM protein: implications for plasmid DNA segregation.The Journal of biological chemistry · 2010Article
- In vitro assembly studies of FtsZ/tubulin-like proteins (TubZ) from Bacillus plasmids: evidence for a capping mechanism.The Journal of biological chemistry · 2008Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
We recently identified a minireplicon of pBtoxis from Bacillus thuringiensis subsp. israelensis that contained an operon encoding two novel proteins (ORF156 and ORF157), both of which are required for replication. ORF157 contains a helix-turn-helix motif and shares no homology with known plasmid replication proteins (Rep), and ORF156 contains the signature motif present in FtsZ/tubulin proteins, the latter of which are known to function in cell division and chromosome segregation. Here we show that the minimal sequence composed of four 12-bp imperfect direct repeats (iterons) in the pBtoxis minireplicon was sufficient to replicate a reporter plasmid in B. thuringiensis subsp. israelensis when ORF156 and ORF157 functions were provided in trans. To further investigate the roles of ORF156 and ORF157 in pBtoxis replication, six-histidine-tagged recombinant rORF156 and rORF157 proteins were purified from Escherichia coli and used in electrophoretic mobility shift assays. Our results demonstrated that rORF157, but not rORF156, binds specifically to the pBtoxis iterons, suggesting that ORF157 functions as a Rep protein. Although rORF156 did not bind to the iteron sequence, we showed that it bound to rORF157-DNA complexes. In addition, we showed that rORF156 has GTPase activity characteristic of the FtsZ/tubulin superfamily of proteins. Taken together, these results suggest that the iterons compose the minimal replication origin (ori) of pBtoxis and that ORF157 and ORF156 are involved in the initiation of pBtoxis replication and possibly in the segregation and partitioning of this plasmid to daughter cells.
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