ArticleThe Journal of molecular diagnostics : JMD2007
A highly sensitive, multiplex broad-spectrum PCR-DNA-enzyme immunoassay and reverse hybridization assay for rapid detection and identification of Chlamydia trachomatis serovars.
Article in The Journal of molecular diagnostics : JMD, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 51 citations in OpenAlex.
- Identifying the Impact ofPathogens (Basel, Switzerland) · 2024Article
- Identification and characterization of mixed infections ofFrontiers in microbiology · 2022Article
- An Asymptomatic Patient with Fatal Infertility Carried a Swedish Strain ofMicroorganisms · 2019Article
- Comparison of reverse hybridization and ompA sequencing methods applied on Chlamydia trachomatis strains from Tunisia.MicrobiologyOpen · 2018Article
- First Detection ofThe open microbiology journal · 2018Article
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- Unveiling the Multilocus Sequence Typing (MLST) Schemes and Core Genome Phylogenies for GenotypingFrontiers in microbiology · 2018Article
- High Frequency of Chlamydia trachomatis Mixed Infections Detected by Microarray Assay in South American Samples.PloS one · 2016Article
- Human and Pathogen Factors Associated with Chlamydia trachomatis-Related Infertility in Women.Clinical microbiology reviews · 2015Review
- Serogroup distribution of urogenital Chlamydia trachomatis in urban ethnic groups in The Netherlands.Epidemiology and infection · 2014Article
- Serovar D and E of serogroup B induce highest serological responses in urogenital Chlamydia trachomatis infections.BMC infectious diseases · 2014Article
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- Chlamydia trachomatis genovar distribution in clinical urogenital specimens from Tunisian patients: high prevalence of C. trachomatis genovar E and mixed infections.BMC infectious diseases · 2012Article
- Anal infections with concomitant Chlamydia trachomatis genotypes among men who have sex with men in Amsterdam, the Netherlands.BMC infectious diseases · 2011Article
- Evaluation of a novel Chlamydia trachomatis microsphere suspension assay for detection and genotyping of the different serovars in clinical samples.The Journal of molecular diagnostics : JMD · 2011Article
- Chlamydia trachomatis and risk of prevalent and incident cervical premalignancy in a population-based cohort.Journal of the National Cancer Institute · 2010Article
- Development and evaluation of an ompA quantitative real-time PCR assay for Chlamydia trachomatis serovar determination.Journal of clinical microbiology · 2010Article
- Vertical transmission of Chlamydia trachomatis in Chongqing China.Current microbiology · 2009Article
- Evaluation of a novel PCR-based assay for detection and identification of Chlamydia trachomatis serovars in cervical specimens.Journal of clinical microbiology · 2007Article
Corrections and comments
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Authors and funding
11 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chlamydia trachomatis (Ct) comprises distinct serogroups and serovars. The present study evaluates a novel Ct amplification, detection, and genotyping method (Ct-DT assay). The Ct-DT amplification step is a multiplex broad-spectrum PCR for the cryptic plasmid and the VD2-region of ompl. The Ct-DT detection step involves a DNA enzyme immunoassay (DEIA) using probes for serogroups (group B, C, and intermediate) and the cryptic plasmid, permitting sensitive detection of 19 Ct serovars (A, B/Ba, C, D/Da, E, F, G/Ga, H, I/Ia, J, K, L1, L2/L2a, and L3) without any cross-reactivity with other Chlamydia species and pathogenic bacteria or commensal organisms of the genital tract. Ct-positive samples are analyzed by a nitrocellulose-based reverse hybridization assay (RHA) containing probes for the 19 different serovars and for the cryptic plasmid. The sensitivity of the PCR-DEIA on clinical specimen is equivalent to that of the Cobas TaqMan assay [kappa = 0.95 (95% confidence interval = 0.92 to 0.99)]. Using the RHA, 98% of the Ct-DT detection step-positive samples could be typed. Analysis of cervical swabs from Uganda and The Netherlands revealed that the most common serovars in Uganda are G/Ga (45%), E (21%), K (13%), and F (8%), and in The Netherlands serovars E (38%), F (23%), G/Ga (11%), and D/Da (7%) were most common. Thus, multiplex broad-spectrum PCR in combination with DEIA and RHA permits highly sensitive and specific detection and identification of Ct serovars.
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