ArticleBiophysical journal2008
Exploring transferrin-receptor interactions at the single-molecule level.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 54 citations in OpenAlex.
- Targeting Transferrin Receptor 1 for Enhancing Drug Delivery Through the Blood-Brain Barrier for Alzheimer's Disease.International journal of molecular sciences · 2025Review
- Valency of Ligand-Receptor Binding from Pair Potentials.Journal of chemical theory and computation · 2024Article
- IST1 regulates select recycling pathways.Traffic (Copenhagen, Denmark) · 2024Article
- Duodenal macrophages control dietary iron absorption via local degradation of transferrin.Blood · 2023Article
- Nanomechanical Study of Enzyme: Coenzyme Complexes: Bipartite Sites in Plastidic Ferredoxin-NADPAntioxidants (Basel, Switzerland) · 2022Article
- Entry modes of ellipsoidal nanoparticles on a membrane during clathrin-mediated endocytosis.Soft matter · 2019Article
- Stochastic modeling of nanoparticle internalization and expulsion through receptor-mediated transcytosis.Nanoscale · 2019Article
- Stochastic simulations of nanoparticle internalization through transferrin receptor dependent clathrin-mediated endocytosis.Biochimica et biophysica acta. General subjects · 2018Article
- Improving estimation of kinetic parameters in dynamic force spectroscopy using cluster analysis.The Journal of chemical physics · 2018Article
- Single-molecule dynamics and mechanisms of metalloregulators and metallochaperones.Biochemistry · 2013Review
- Exploring the energy profile of human IgG/rat anti-human IgG interactions by dynamic force spectroscopy.The protein journal · 2012Article
- Transferrin as a model system for method development to study structure, dynamics and interactions of metalloproteins using mass spectrometry.Biochimica et biophysica acta · 2012Review
- Interaction between pheromone and its receptor of the fission yeast Schizosaccharomyces pombe examined by a force spectroscopy study.Journal of biomedicine & biotechnology · 2012Article
- A role for septins in the interaction between the Listeria monocytogenes INVASION PROTEIN InlB and the Met receptor.Biophysical journal · 2011Article
- Direct detection of cellular adaptation to local cyclic stretching at the single cell level by atomic force microscopy.Biophysical journal · 2011Article
- Noncanonical interactions between serum transferrin and transferrin receptor evaluated with electrospray ionization mass spectrometry.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Characterization of the interaction between diferric transferrin and transferrin receptor 2 by functional assays and atomic force microscopy.Journal of molecular biology · 2010Article
- Article
- Existence of a noncanonical state of iron-bound transferrin at endosomal pH revealed by hydrogen exchange and mass spectrometry.Journal of molecular biology · 2009Article
- Profiling TRA-1-81 antigen distribution on a human embryonic stem cell.Biochemical and biophysical research communications · 2008Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Interaction between the iron transporter protein transferrin (Tf) and its receptor at the cell surface is fundamental for most living organisms. Tf receptor (TfR) binds iron-loaded Tf (holo-Tf) and transports it to endosomes, where acidic pH favors iron release. Iron-free Tf (apo-Tf) is then brought back to the cell surface and dissociates from TfR. Here we investigated the Tf-TfR interaction at the single-molecule level under different conditions encountered during the Tf cycle. An atomic force microscope tip functionalized with holo-Tf or apo-Tf was used to probe TfR. We tested both purified TfR anchored to a mica substrate and in situ TfR at the surface of living cells. Dynamic force measurements showed similar results for TfR on mica or at the cell surface but revealed striking differences between holo-Tf-TfR and apo-Tf-TfR interactions. First, the forces necessary to unbind holo-Tf and TfR are always stronger compared to the apo-Tf-TfR interaction. Second, dissociation of holo-Tf-TfR complex involves overcoming two energy barriers, whereas the apo-Tf-TfR unbinding pathway comprises only one energy barrier. These results agree with a model that proposes differences in the contact points between holo-Tf-TfR and apo-Tf-TfR interactions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.