Evidence map›Paper›PMID 17870469›Full record

ReviewCurrent opinion in structural biology2007

Yeast surface display for protein engineering and characterization.

S Annie Gai, K Dane Wittrup

Abstract readReview
In one paragraph

Review in Current opinion in structural biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 181 papers.

0numbers the graph read from it
0cells of the map it votes in
181citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

181 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. High-throughput screening for class I peptide MHC binding via yeast surface display.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  6. Article
  7. ADAPT-M: A workflow for rapid, quantitativebioRxiv : the preprint server for biology · 2025
    Article
  8. Article
  9. Article
  10. Article
  11. Structure-guided engineering of CD112 receptor variants for optimized immunotherapy.Molecular therapy : the journal of the American Society of Gene Therapy · 2025
    Article
  12. Article
  13. Review
  14. Construction of L-type lectin displaying Saccharomyces cerevisiae for Vibrio parahaemolyticus agglutination.International microbiology : the official journal of the Spanish Society for Microbiology · 2025
    Article
  15. Review
  16. Review
  17. Multiplex, multimodal mapping of variant effects in secreted proteins.bioRxiv : the preprint server for biology · 2025
    Article
  18. Article
  19. Review
  20. Review

121 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

S Annie GaiDepartment of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Room E19-563, Cambridge, MA 02139, USA.
K Dane Wittrup

Funding

Engineered Antibody EGFR Antagonist Cancer TherapeuticsR01CA096504 · NCI · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI WITTRUP, KARL DANE · 2002 to 2017
$9.7M
Foundations of Pretargeted RadioimmunotherapyR01CA101830 · NCI · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI WITTRUP, KARL DANE · 2005 to 2014
$2.7M
Engineering and Analysis of T cell CD3 and IL2R SignalsR01AI065824 · NIAID · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI WITTRUP, KARL DANE · 2005 to 2008
$2.2M
NCI NIH HHS CA101830NCI NIH HHS R01 CA096504NCI NIH HHS R01 CA101830NIAID NIH HHS AI065824NIAID NIH HHS R01 AI065824
6 · The paper itself

Abstract

Yeast surface display is being employed to engineer desirable properties into proteins for a broad variety of applications. Labeling with soluble ligands enables rapid and quantitative analysis of yeast-displayed libraries by flow cytometry, while cell-surface selections allow screening of libraries with insoluble or even as-yet-uncharacterized binding targets. In parallel, the utilization of yeast surface display for protein characterization, including in particular the mapping of functional epitopes mediating protein-protein interactions, represents a significant recent advance.

Indexed as

AnimalsEnzymes, ImmobilizedHumansPeptide LibraryProtein ConformationProtein EngineeringProtein Interaction MappingSaccharomyces cerevisiaeSolubilityEnzymes, ImmobilizedPeptide Library

Identifiers

PMID17870469
PMCPMC4038029

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.