ArticleJournal of molecular biology2007
A switch in the mechanism of communication between the two DNA-binding sites in the SfiI restriction endonuclease.
Article in Journal of molecular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Identification of Nanobodies Blocking Intimate Adherence of Shiga Toxin-Producing Escherichia coli to Epithelial Cells.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Structure of 5-hydroxymethylcytosine-specific restriction enzyme, AbaSI, in complex with DNA.Nucleic acids research · 2014Article
- Organization of the BcgI restriction-modification protein for the cleavage of eight phosphodiester bonds in DNA.Nucleic acids research · 2013Article
- Visual analysis of concerted cleavage by type IIF restriction enzyme SfiI in subsecond time region.Biophysical journal · 2011Article
- Concerted action at eight phosphodiester bonds by the BcgI restriction endonuclease.Nucleic acids research · 2011Article
- Generation and characterization of the human neutralizing antibody fragment Fab091 against rabies virus.Acta pharmacologica Sinica · 2011Article
- Domain swapping in allosteric modulation of DNA specificity.PLoS biology · 2010Article
- DNA synapsis through transient tetramerization triggers cleavage by Ecl18kI restriction enzyme.Nucleic acids research · 2010Article
- The unstructured C-terminal extension of UvrD interacts with UvrB, but is dispensable for nucleotide excision repair.DNA repair · 2009Article
- Differences between Ca2+ and Mg2+ in DNA binding and release by the SfiI restriction endonuclease: implications for DNA looping.Nucleic acids research · 2009Article
- Dissecting protein-induced DNA looping dynamics in real time.Nucleic acids research · 2009Article
- Targeting individual subunits of the FokI restriction endonuclease to specific DNA strands.Nucleic acids research · 2009Article
- Dynamics and consequences of DNA looping by the FokI restriction endonuclease.Nucleic acids research · 2008Article
- Tetrameric restriction enzymes: expansion to the GIY-YIG nuclease family.Nucleic acids research · 2008Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
While many Type II restriction enzymes are dimers with a single DNA-binding cleft between the subunits, SfiI is a tetramer of identical subunits. Two of its subunits (a dimeric unit) create one DNA-binding cleft, and the other two create a second cleft on the opposite side of the protein. The two clefts bind specific DNA cooperatively to give a complex of SfiI with two recognition sites. This complex is responsible for essentially all of the DNA-cleavage reactions by SfiI: virtually none is due to the complex with one site. The communication between the DNA-binding clefts was examined by disrupting one of the very few polar interactions in the otherwise hydrophobic interface between the dimeric units: a tyrosine hydroxyl was removed by mutation to phenylalanine. The mutant protein remained tetrameric in solution and could bind two DNA sites. But instead of being activated by binding two sites, like wild-type SfiI, it showed maximal activity when bound to a single site and had a lower activity when bound to two sites. This interaction across the dimer interface thus enforces in wild-type SfiI a cooperative transition between inactive and active states in both dimers, but without this interaction as in the mutant protein, a single dimer can undergo the transition to give a stable intermediate with one inactive dimer and one active dimer.
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