ArticleJournal of clinical microbiology2007
Novel approach for genotyping varicella-zoster virus strains from Germany.
Article in Journal of clinical microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- [S2k guideline for the diagnosis and therapy of zoster and post-zoster neuralgia].GMS infectious diseases · 2020Article
- Sequencing of 21 varicella-zoster virus genomes reveals two novel genotypes and evidence of recombination.Journal of virology · 2012Article
- Monitoring prevalence of varicella-zoster virus clades in Germany.Medical microbiology and immunology · 2011Article
- Molecular analysis of varicella-zoster virus strains circulating in Tanzania demonstrating the presence of genotype M1.Journal of clinical microbiology · 2008Article
- In vitro replication of varicella-zoster virus in human retinal pigment epithelial cells.Journal of clinical microbiology · 2008Article
- Novel varicella-zoster virus glycoprotein E gene mutations associated with genotypes A and D.Journal of clinical microbiology · 2008Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, we present a novel genotyping scheme to classify German wild-type varicella-zoster virus (VZV) strains and to differentiate them from the Oka vaccine strain (genotype B). This approach is based on analysis of four loci in open reading frames (ORFs) 51 to 58, encompassing a total length of 1,990 bp. The new genotyping scheme produced identical clusters in phylogenetic analyses compared to full-genome sequences from well-characterized VZV strains. Based on genotype A, D, B, and C reference strains, a dichotomous identification key (DIK) was developed and applied for VZV strains obtained from vesicle fluid and liquor samples originating from 42 patients suffering from varicella or zoster between 2003 and 2006. Sequencing of regions in ORFs 51, 52, 53, 56, 57, and 58 identified 18 single-nucleotide polymorphisms (SNPs), including two novel ones, SNP 89727 and SNP 92792 in ORF51 and ORF52, respectively. The DIK as well as phylogenetic analysis by Bayesian inference showed that 14 VZV strains belonged to genotype A, and 28 VZV strains were classified as genotype D. Neither Japanese (vaccine)-like B strains nor recombinant-like C strains were found within the samples from Germany. The novel genotyping scheme and the DIK were demonstrated to be practical and simple and allow the highly efficient replication of phylogenetic patterns in VZV initially derived from full-genome DNA sequence analyses. Therefore, this approach may allow us to draw a more comprehensive picture of wild-type VZV strains circulating in Germany and Central Europe by high-throughput procedures in the future.
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