ArticleNucleic acids research2007
Genomic profiling of CpG methylation and allelic specificity using quantitative high-throughput mass spectrometry: critical evaluation and improvements.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT02623400 (The Effect of Perinatal Stress on the Development of Preterm Infants), which is not on this map. Cited by 124 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
The Effect of Perinatal Stress on the Development of Preterm Infants
Who cites it
124 citing papers in PubMed.
- Hypermethylation at loci sensitive to the prenatal environment is associated with increased incidence of myocardial infarction.International journal of epidemiology · 2012Trial
- Functional analysis of 5-lipoxygenase promoter repeat variants.Human molecular genetics · 2009Trial
- Single-Base Resolution DNA Methylation Analysis Through Targeted Locus-Specific Bisulfite Sequencing in Plants.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Use of Multiple Machine Learning Approaches for Selecting Urothelial Cancer-Specific DNA Methylation Biomarkers in Urine.International journal of molecular sciences · 2024Article
- Predictive value of DNA methylation patterns in AML patients treated with an azacytidine containing induction regimen.Clinical epigenetics · 2023Article
- Association between academic pressure, NR3C1 gene methylation, and anxiety symptoms among Chinese adolescents: a nested case-control study.BMC psychiatry · 2023Article
- Next-Generation Bisulfite Sequencing for Targeted DNA Methylation Analysis.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Current and Emerging Technologies for the Analysis of the Genome-Wide and Locus-Specific DNA Methylation Patterns.Advances in experimental medicine and biology · 2022Article
- Leptin Methylation and mRNA Expression Associated With Psychopathology in Schizophrenia Inpatients.Frontiers in psychiatry · 2022Article
- A multi-modal MRI analysis of brain structure and function in relation to OXT methylation in maltreated children and adolescents.Translational psychiatry · 2021Article
- Associations of FKBP5 polymorphisms and methylation and parenting style with depressive symptoms among Chinese adolescents.BMC psychiatry · 2021Article
- Pathways linking adverse environments to emerging adults' substance abuse and depressive symptoms: A prospective analysis of rural African American men.Development and psychopathology · 2021Article
- Epigenetic Mediation ofBrain sciences · 2021Article
- Regulation of impulsive and aggressive behaviours by a novel lncRNA.Molecular psychiatry · 2021Article
- DNA Methylation in Solid Tumors: Functions and Methods of Detection.International journal of molecular sciences · 2021Review
- Methylation of OXT and OXTR genes, central oxytocin, and social behavior in female macaques.Hormones and behavior · 2020Article
- In-Vitro Identification and In-Vivo Confirmation of DNA Methylation Biomarkers for Urothelial Cancer.Biomedicines · 2020Article
- 18q12.3-q21.1 microdeletion detected in the prenatally alcohol-exposed dizygotic twin with discordant fetal alcohol syndrome phenotype.Molecular genetics & genomic medicine · 2020Article
- Differential methylation pattern of xenobiotic metabolizing genes and susceptibility to Balkan endemic nephropathy, in a cohort of Romanian patients.Journal of nephrology · 2020Article
- Stomatin plays a suppressor role in non-small cell lung cancer metastasis.Chinese journal of cancer research = Chung-kuo yen cheng yen chiu · 2019Article
64 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CpG methylation is a key component of the epigenome architecture that is associated with changes in gene expression without a change to the DNA sequence. Since the first reports on deregulation of DNA methylation, in diseases such as cancer, and the initiation of the Human Epigenome Project, an increasing need has arisen for a detailed, high-throughput and quantitative method of analysis to discover and validate normal and aberrant DNA methylation profiles in large sample cohorts. Here we present an improved protocol using base-specific fragmentation and MALDI-TOF mass spectrometry that enables a sensitive and high-throughput method of DNA methylation analysis, quantitative to 5% methylation for each informative CpG residue. We have determined the accuracy, variability and sensitivity of the protocol, implemented critical improvements in experimental design and interpretation of the data and developed a new formula to accurately measure CpG methylation. Key innovations now permit determination of differential and allele-specific methylation, such as in cancer and imprinting. The new protocol is ideally suitable for detailed DNA methylation analysis of multiple genomic regions and large sample cohorts that is critical for comprehensive profiling of normal and diseased human epigenomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.