ArticleThe Journal of clinical investigation2007
Targeted gene deletion in Candida parapsilosis demonstrates the role of secreted lipase in virulence.
Article in The Journal of clinical investigation, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
69 citing papers in PubMed, 1 synthesis or guideline pooled it, 150 citations in OpenAlex.
- Candida parapsilosis is a significant neonatal pathogen: a systematic review and meta-analysis.The Pediatric infectious disease journal · 2013Pooled it
- Current Insights intoJournal of fungi (Basel, Switzerland) · 2025Article
- PCR-based CRISPR/Cas9 system for fluorescent tagging: A tool for studying Candida parapsilosis virulence.PloS one · 2025Article
- Shining a light onmSphere · 2024Article
- Phenotypic and Genotypic Characterization of Candida parapsilosis complex isolates from a Lebanese Hospital.Research square · 2024Article
- Mycological evaluation of frozen meat with special reference to yeasts.Veterinary world · 2023Article
- Review
- Contribution ofInfection and drug resistance · 2023Article
- Proteomic Analysis ofPathogens (Basel, Switzerland) · 2022Article
- Recognition of Cell Wall Mannosylated Components as a Conserved Feature for Fungal Entrance, Adaptation and Survival Within Trophozoites ofFrontiers in cellular and infection microbiology · 2022Article
- Update on the Pathogenesis, Virulence, and Treatment ofPathogens & immunity · 2022Review
- Enhancing the chemical transformation ofVirulence · 2021Article
- The gut commensal fungus, Candida parapsilosis, promotes high fat-diet induced obesity in mice.Communications biology · 2021Article
- Genetic Manipulation as a Tool to UnravelJournal of fungi (Basel, Switzerland) · 2021Review
- Virulence of ClinicalPathogens (Basel, Switzerland) · 2021Article
- AJournal of fungi (Basel, Switzerland) · 2021Article
- Article
- A decade after the emergence of Candida auris: what do we know?European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2020Review
- Identification and Characterization of a Neutral Locus for Knock-in Purposes inFrontiers in microbiology · 2020Article
- Reduced phagocytosis and killing of Cryptococcus neoformans biofilm-derived cells by J774.16 macrophages is associated with fungal capsular production and surface modification.Fungal genetics and biology : FG & B · 2019Article
9 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
Candida parapsilosis is a major cause of human disease, yet little is known about the pathogen's virulence. We have developed an efficient gene deletion system for C. parapsilosis based on the repeated use of the dominant nourseothricin resistance marker (caSAT1) and its subsequent deletion by FLP-mediated, site-specific recombination. Using this technique, we deleted the lipase locus in the C. parapsilosis genome consisting of adjacent genes CpLIP1 and CpLIP2. Additionally we reconstructed the CpLIP2 gene, which restored lipase activity. Lipolytic activity was absent in the null mutants, whereas the WT, heterozygous, and reconstructed mutants showed similar lipase production. Biofilm formation was inhibited with lipase-negative mutants and their growth was significantly reduced in lipid-rich media. The knockout mutants were more efficiently ingested and killed by J774.16 and RAW 264.7 macrophage-like cells. Additionally, the lipase-negative mutants were significantly less virulent in infection models that involve inoculation of reconstituted human oral epithelium or murine intraperitoneal challenge. These studies represent what we believe to be the first targeted disruption of a gene in C. parapsilosis and show that C. parapsilosis-secreted lipase is involved in disease pathogenesis. This efficient system for targeted gene deletion holds great promise for rapidly enhancing our knowledge of the biology and virulence of this increasingly common invasive fungal pathogen.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.