ArticleTransgenic research2008
Development of an in planta method for transformation of alfalfa (Medicago sativa).
Article in Transgenic research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Improved Protocol for EfficientPlants (Basel, Switzerland) · 2024Article
- A comprehensive review of in planta stable transformation strategies.Plant methods · 2024Review
- Plant Promoters and Terminators for High-Precision Bioengineering.Biodesign research · 2023Review
- Cisgenics and intragenics: boon or bane for crop improvement.Frontiers in plant science · 2023Review
- Genetic Improvement of Wheat for Drought Tolerance: Progress, Challenges and Opportunities.Plants (Basel, Switzerland) · 2022Review
- The Intragenesis and Synthetic Biology Approach towards Accelerating Genetic Gains on Strawberry: Development of New Tools to Improve Fruit Quality and Resistance to Pathogens.Plants (Basel, Switzerland) · 2021Article
- Establishment of a Novel and EfficientPlants (Basel, Switzerland) · 2021Article
- Article
- Plant 3' Regulatory Regions From mRNA-Encoding Genes and Their Uses to Modulate Expression.Frontiers in plant science · 2020Review
- Precision breeding for RNAi suppression of a major 4-coumarate:coenzyme A ligase gene improves cell wall saccharification from field grown sugarcane.Plant molecular biology · 2016Article
- Genetically modified (GM) crops: milestones and new advances in crop improvement.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2016Review
- Co-expression of tonoplast Cation/H(+) antiporter and H(+)-pyrophosphatase from xerophyte Zygophyllum xanthoxylum improves alfalfa plant growth under salinity, drought and field conditions.Plant biotechnology journal · 2016Article
- ZxNHX controls Na⁺ and K⁺ homeostasis at the whole-plant level in Zygophyllum xanthoxylum through feedback regulation of the expression of genes involved in their transport.Annals of botany · 2015Article
- Co-transforming bar and CsALDH Genes Enhanced Resistance to Herbicide and Drought and Salt Stress in Transgenic Alfalfa (Medicago sativa L.).Frontiers in plant science · 2015Article
- Co-expression of xerophyte Zygophyllum xanthoxylum ZxNHX and ZxVP1-1 confers enhanced salinity tolerance in chimeric sugar beet (Beta vulgaris L.).Frontiers in plant science · 2015Article
- Genomics-assisted breeding in four major pulse crops of developing countries: present status and prospects.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2014Review
- An efficient in planta transformation of Jatropha curcas (L.) and multiplication of transformed plants through in vivo grafting.Protoplasma · 2014Article
- Agrobacterium tumefaciens-mediated in planta seed transformation strategy in sugarcane.Plant cell reports · 2013Article
- Assessment of simple marker-free genetic transformation techniques in alfalfa.Plant cell reports · 2011Article
- An efficient method for the production of marker-free transgenic plants of peanut (Arachis hypogaea L.).Plant cell reports · 2010Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Conventional methods in transforming alfalfa (Medicago sativa) require multiple tissue culture manipulations that are time-consuming and expensive, while applicable only to a few highly regenerable genotypes. Here, we describe a simple in planta method that makes it possible to transform a commercial variety without employing selectable marker genes. Basically, young seedlings are cut at the apical node, cold-treated, and vigorously vortexed in an Agrobacterium suspension also containing sand. About 7% of treated seedlings produced progenies segregating for the T-DNA. The vortex-mediated seedling transformation method was applied to transform alfalfa with an all-native transfer DNA comprising a silencing construct for the caffeic acid o-methyltransferase (Comt) gene. Resulting intragenic plants accumulated reduced levels of the indigestible fiber component lignin that lowers forage quality. The absence of both selectable marker genes and other foreign genetic elements may expedite the governmental approval process for quality-enhanced alfalfa.
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