ArticleProceedings of the National Academy of Sciences of the United States of America2007
Dual-specificity splice sites function alternatively as 5' and 3' splice sites.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 50 citations in OpenAlex.
- RNA-Based Therapies for Inherited Metabolic Disorders.Journal of inherited metabolic disease · 2026Review
- High-efficiency base editing in the retina in primates and human tissues.Nature medicine · 2025Article
- The unusual gene architecture of polyubiquitin is created by dual-specific splice sites.Genome biology · 2024Article
- Stargardt disease-associated missense and synonymous ABCA4 variants result in aberrant splicing.Human molecular genetics · 2023Article
- MEN1 is a regulator of alternative splicing and prevents R-loop-induced genome instability through suppression of RNA polymerase II elongation.Nucleic acids research · 2023Article
- Recursive splicing discovery using lariats in total RNA sequencing.Life science alliance · 2023Article
- Article
- Cutting a Long Intron Short: Recursive Splicing and Its Implications.Frontiers in physiology · 2016Article
- Splicing of many human genes involves sites embedded within introns.Nucleic acids research · 2015Article
- Article
- OLego: fast and sensitive mapping of spliced mRNA-Seq reads using small seeds.Nucleic acids research · 2013Article
- Re-splicing of mature mRNA in cancer cells promotes activation of distant weak alternative splice sites.Nucleic acids research · 2012Article
- Widespread recognition of 5' splice sites by noncanonical base-pairing to U1 snRNA involving bulged nucleotides.Genes & development · 2012Article
- An RNA architectural locus control region involved in Dscam mutually exclusive splicing.Nature communications · 2012Article
- Intron definition and a branch site adenosine at nt 385 control RNA splicing of HPV16 E6*I and E7 expression.PloS one · 2012Article
- SpliceTrap: a method to quantify alternative splicing under single cellular conditions.Bioinformatics (Oxford, England) · 2011Article
- SON controls cell-cycle progression by coordinated regulation of RNA splicing.Molecular cell · 2011Article
- Allele-specific recognition of the 3' splice site of INS intron 1.Human genetics · 2010Article
- Single molecule analysis of combinatorial splicing.Nucleic acids research · 2010Article
- Defining the regulatory network of the tissue-specific splicing factors Fox-1 and Fox-2.Genes & development · 2008Article
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
As a result of large-scale sequencing projects and recent splicing-microarray studies, estimates of mammalian genes expressing multiple transcripts continue to increase. This expansion of transcript information makes it possible to better characterize alternative splicing events and gain insights into splicing mechanisms and regulation. Here, we describe a class of splice sites that we call dual-specificity splice sites, which we identified through genome-wide, high-quality alignment of mRNA/EST and genome sequences and experimentally verified by RT-PCR. These splice sites can be alternatively recognized as either 5' or 3' splice sites, and the dual splicing is conceptually similar to a pair of mutually exclusive exons separated by a zero-length intron. The dual-splice-site sequences are essentially a composite of canonical 5' and 3' splice-site consensus sequences, with a CAG|GURAG core. The relative use of a dual site as a 5' or 3' splice site can be accurately predicted by assuming competition for specific binding between spliceosomal components involved in recognition of 5' and 3' splice sites, respectively. Dual-specificity splice sites exist in human and mouse, and possibly in other vertebrate species, although most sites are not conserved, suggesting that their origin is recent. We discuss the implications of this unusual splicing pattern for the diverse mechanisms of exon recognition and for gene evolution.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.