ArticleThe Biochemical journal2008
Genomic location of the human RNA polymerase II general machinery: evidence for a role of TFIIF and Rpb7 at both early and late stages of transcription.
Article in The Biochemical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 35 citations in OpenAlex.
- Mechanisms and Functions of the RNA Polymerase II General Transcription Machinery during the Transcription Cycle.Biomolecules · 2024Review
- Specialization of RNA Polymerase I in Comparison to Other Nuclear RNA Polymerases of Saccharomyces cerevisiae.Methods in molecular biology (Clifton, N.J.) · 2022Review
- The comparison of transcriptomic response of green microalga Chlorella sorokiniana exposure to environmentally relevant concentration of cadmium(II) and 4-n-nonylphenol.Environmental geochemistry and health · 2020Article
- Nucleosomal Barrier to Transcription: Structural Determinants and Changes in Chromatin Structure.Biochemistry & molecular biology journal · 2016Article
- Transcription factors TFIIF and TFIIS promote transcript elongation by RNA polymerase II by synergistic and independent mechanisms.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Transcription factors IIS and IIF enhance transcription efficiency by differentially modifying RNA polymerase pausing dynamics.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Activation and reactivation of the RNA polymerase II trigger loop for intrinsic RNA cleavage and catalysis.Transcription · 2014Article
- RNA polymerase II transcription elongation control.Chemical reviews · 2013Review
- Quantitative proteomics demonstrates that the RNA polymerase II subunits Rpb4 and Rpb7 dissociate during transcriptional elongation.Molecular & cellular proteomics : MCP · 2013Article
- RNA polymerase II elongation control.Annual review of biochemistry · 2012Review
- The functions of TFIIF during initiation and transcript elongation are differentially affected by phosphorylation by casein kinase 2.The Journal of biological chemistry · 2011Article
- Efficient and rapid nucleosome traversal by RNA polymerase II depends on a combination of transcript elongation factors.The Journal of biological chemistry · 2011Article
- Transcription factor IIS cooperates with the E3 ligase UBR5 to ubiquitinate the CDK9 subunit of the positive transcription elongation factor B.The Journal of biological chemistry · 2011Article
- Highly reproducible label free quantitative proteomic analysis of RNA polymerase complexes.Molecular & cellular proteomics : MCP · 2011Article
- Identification of proteins associated with the yeast mitochondrial RNA polymerase by tandem affinity purification.Yeast (Chichester, England) · 2009Article
- Promoter proximal pausing on genes in metazoans.Chromosoma · 2009Review
- Genome-associated RNA polymerase II includes the dissociable Rpb4/7 subcomplex.The Journal of biological chemistry · 2008Article
- Genomewide recruitment analysis of Rpb4, a subunit of polymerase II in Saccharomyces cerevisiae, reveals its involvement in transcription elongation.Eukaryotic cell · 2008Article
- Steps towards a repertoire of comprehensive maps of human protein interaction networks: the Human Proteotheque Initiative (HuPI).Biochemistry and cell biology = Biochimie et biologie cellulaire · 2008Review
- The Rpb4 subunit of RNA polymerase II contributes to cotranscriptional recruitment of 3' processing factors.Molecular and cellular biology · 2008Article
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
The functions ascribed to the mammalian GTFs (general transcription factors) during the various stages of the RNAPII (RNA polymerase II) transcription reaction are based largely on in vitro studies. To gain insight as to the functions of the GTFs in living cells, we have analysed the genomic location of several human GTF and RNAPII subunits carrying a TAP (tandem-affinity purification) tag. ChIP (chromatin immunoprecipitation) experiments using anti-tag beads (TAP-ChIP) allowed the systematic localization of the tagged factors. Enrichment of regions located close to the TIS (transcriptional initiation site) versus further downstream TRs (transcribed regions) of nine human genes, selected for the minimal divergence of their alternative TIS, were analysed by QPCR (quantitative PCR). We show that, in contrast with reports using the yeast system, human TFIIF (transcription factor IIF) associates both with regions proximal to the TIS and with further downstream TRs, indicating an in vivo function in elongation for this GTF. Unexpectedly, we found that the Rpb7 subunit of RNAPII, known to be required only for the initiation phase of transcription, remains associated with the polymerase during early elongation. Moreover, ChIP experiments conducted under stress conditions suggest that Rpb7 is involved in the stabilization of transcribing polymerase molecules, from initiation to late elongation stages. Together, our results provide for the first time a general picture of GTF function during the RNAPII transcription reaction in live mammalian cells and show that TFIIF and Rpb7 are involved in both early and late transcriptional stages.
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