Evidence map›Paper›PMID 17846325›Full record

ArticleCirculation2007

Differentiation, survival, and function of embryonic stem cell derived endothelial cells for ischemic heart disease.

Zongjin Li, Jenny C Wu, Ahmad Y Sheikh, Daniel Kraft, Feng Cao, Xiaoyan Xie, Manishkumar Patel, Sanjiv S Gambhir, Robert C Robbins, John P Cooke and 1 more

Open access · bronzeAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Circulation, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 106 papers.

0numbers the graph read from it
0cells of the map it votes in
106citing papers in PubMed
9.5field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

106 citing papers in PubMed, 218 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Review
  5. Review
  6. Review
  7. Review
  8. Article
  9. Article
  10. Article
  11. Review
  12. Article
  13. Article
  14. Generation of Endothelial Cells From Human Pluripotent Stem Cells.Arteriosclerosis, thrombosis, and vascular biology · 2019
    Review
  15. Review
  16. Article
  17. Review
  18. Article
  19. Article
  20. Article

46 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

Zongjin LiDepartment of Radiology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Jenny C Wu
Ahmad Y Sheikh
Daniel Kraft
Feng Cao
Xiaoyan Xie
Manishkumar Patel
Sanjiv S Gambhir
Robert C Robbins
John P Cooke
Joseph C Wu
Stanford University · US

Funding

Biological Insights into Dynamics of Stem Cell Differentiation and MisbehaviorR21HL091453 · NHLBI · STANFORD UNIVERSITY · PI WU, JOSEPH C. · 2008 to 2009
$719k
Molecular Imaging of Cardiac Cell TransplantationK08HL074883 · NHLBI · STANFORD UNIVERSITY · PI WU, JOSEPH C. · 2005 to 2008
$531k
NHLBI NIH HHS K08 HL074883NHLBI NIH HHS R21 HL091453
6 · The paper itself

Abstract

backgroundEmbryonic stem (ES) cells are distinguished by their capacity for self-renewal and pluripotency. Here we characterize the differentiation of ES cell-derived endothelial cells (ESC-ECs), use molecular imaging techniques to examine their survival in vivo, and determine the therapeutic efficacy of ESC-ECs for restoration of cardiac function after ischemic injury. METHODS AND

resultsMurine ES cells were transfected with a construct composed of a vascular endothelial cadherin promoter driving enhanced green fluorescence protein (pVE-cadherin-eGFP). Differentiation of ES cells to ECs was detected by FACS analysis using Flk-1 (early EC marker at day 4) and VE-cadherin (late EC marker at day 8). After isolation, these ESC-ECs express endothelial cell markers similar to adult mouse lung endothelial cells, form vascular-like channels, and incorporate DiI-labeled acetylated low-density lipoprotein (DiI-Ac-LDL). For in vivo imaging, ES cells were transduced with an ubiquitin promoter driving firefly luciferase and monomeric red fluorescence protein (pUb-Fluc-mRFP). A robust correlation exists between Fluc signals and cell numbers by ex vivo imaging analysis (R2=0.98) and by in vitro enzyme assay (R2=0.94). Afterward, 5x10(5) ESC-ECs or PBS (as control) was injected into the hearts of mice undergoing LAD ligation (n=15 per group). Bioluminescence imaging showed longitudinal survival of transplanted ESC-ECs for approximately 8 weeks. Echocardiogram demonstrated significant functional improvement in the ESC-EC group compared with control (P=0.04). Finally, postmortem analysis confirmed increased presence of small capillaries and venules in the infarcted zones by CD31 staining.

conclusionsThis is the first study to track the fate and function of transplanted ESC-ECs in the heart. With further validation, these ESC-ECs could become a valuable source of cell therapy for induction of angiogenesis in the treatment of myocardial ischemia.

Indexed as

Cell DifferentiationAnimalsCell LineCell SurvivalEmbryonic Stem CellsEndothelial CellsEndothelium, VascularFemaleMiceMyocardial IschemiaStem Cell Transplantation

Identifiers

PMID17846325
PMCPMC3657509
OpenAlexW2169098504

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.