ArticleThe Journal of general physiology2007
The mechanism of fast-gate opening in ClC-0.
Article in The Journal of general physiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 26 citations in OpenAlex.
- Dynamical model of the CLC-2 ion channel reveals conformational changes associated with selectivity-filter gating.PLoS computational biology · 2020Article
- Proton-dependent inhibition, inverted voltage activation, and slow gating of CLC-0 Chloride Channel.PloS one · 2020Article
- Chloride Ion Transport by theFrontiers in chemistry · 2018Article
- Revealing the activation pathway for TMEM16A chloride channels from macroscopic currents and kinetic models.Pflugers Archiv : European journal of physiology · 2016Article
- Gating the glutamate gate of CLC-2 chloride channel by pore occupancy.The Journal of general physiology · 2016Article
- ClC-1 and ClC-2 form hetero-dimeric channels with novel protopore functions.Pflugers Archiv : European journal of physiology · 2014Article
- Conformational changes required for H(+)/Cl(-) exchange mediated by a CLC transporter.Nature structural & molecular biology · 2014Article
- Sequential interaction of chloride and proton ions with the fast gate steer the voltage-dependent gating in ClC-2 chloride channels.The Journal of physiology · 2012Article
- On the mechanism of gating charge movement of ClC-5, a human Cl(-)/H(+) antiporter.Biophysical journal · 2012Article
- Permeant anions contribute to voltage dependence of ClC-2 chloride channel by interacting with the protopore gate.The Journal of physiology · 2010Article
- Paul F. Cranefield Award to Merritt C. Maduke.The Journal of general physiology · 2010Article
- Proton block of the CLC-5 Cl-/H+ exchanger.The Journal of general physiology · 2010Article
- The role of protons in fast and slow gating of the Torpedo chloride channel ClC-0.European biophysics journal : EBJ · 2010Article
- Basis of substrate binding and conservation of selectivity in the CLC family of channels and transporters.Nature structural & molecular biology · 2009Article
- Intracellular proton regulation of ClC-0.The Journal of general physiology · 2008Article
- The role of a conserved lysine in chloride- and voltage-dependent ClC-0 fast gating.The Journal of general physiology · 2007Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
ClC-0 is a chloride channel whose gating is sensitive to both voltage and chloride. Based on analysis of gating kinetics using single-channel recordings, a five-state model was proposed to describe the dependence of ClC-0 fast-gate opening on voltage and external chloride (Chen, T.-Y., and C. Miller. 1996. J. Gen. Physiol. 108:237-250). We aimed to use this five-state model as a starting point for understanding the structural changes that occur during gating. Using macroscopic patch recordings, we were able to reproduce the effects of voltage and chloride that were reported by Chen and Miller and to fit our opening rate constant data to the five-state model. Upon further analysis of both our data and those of Chen and Miller, we learned that in contrast to their conclusions, (a) the features in the data are not adequate to rule out a simpler four-state model, and (b) the chloride-binding step is voltage dependent. In order to be able to evaluate the effects of mutants on gating (described in the companion paper, see Engh et al. on p. 351 of this issue), we developed a method for determining the error on gating model parameters, and evaluated the sources of this error. To begin to mesh the kinetic model(s) with the known CLC structures, a model of ClC-0 was generated computationally based on the X-ray crystal structure of the prokaryotic homolog ClC-ec1. Analysis of pore electrostatics in this homology model suggests that at least two of the conclusions derived from the gating kinetics analysis are consistent with the known CLC structures: (1) chloride binding is necessary for channel opening, and (2) chloride binding to any of the three known chloride-binding sites must be voltage dependent.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.