ArticleMolecular and cellular biology2007
The Rap GTPase activator Drosophila PDZ-GEF regulates cell shape in epithelial migration and morphogenesis.
Article in Molecular and cellular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 44 citations in OpenAlex.
- Rare heterozygous de novo variants in RAPGEF2 are associated with a neurodevelopmental disorder.Genetics in medicine : official journal of the American College of Medical Genetics · 2026Article
- Rap1 regulates lumen continuity via Afadin in renal epithelia.Developmental biology · 2023Article
- Rap1 regulates apical contractility to allow embryonic morphogenesis without tissue disruption and acts in part via Canoe-independent mechanisms.Molecular biology of the cell · 2023Article
- An RNAi Screen for Genes Required for Growth ofG3 (Bethesda, Md.) · 2019Article
- Evolutionary rate covariation analysis of E-cadherin identifies Raskol as a regulator of cell adhesion and actin dynamics in Drosophila.PLoS genetics · 2019Article
- The PDZ-GEF Gef26 regulates synapse development and function via FasII and Rap1 at the Drosophila neuromuscular junction.Experimental cell research · 2019Article
- Rap1 GTPase promotes coordinated collective cell migration in vivo.Molecular biology of the cell · 2018Article
- Rap1, Canoe and Mbt cooperate with Bazooka to promote zonula adherens assembly in the fly photoreceptor.Journal of cell science · 2018Article
- Rap1 acts via multiple mechanisms to position Canoe and adherens junctions and mediate apical-basal polarity establishment.Development (Cambridge, England) · 2018Article
- The Rap activator Gef26 regulates synaptic growth and neuronal survival via inhibition of BMP signaling.Molecular brain · 2017Article
- Pulling together: Tissue-generated forces that drive lumen morphogenesis.Seminars in cell & developmental biology · 2016Review
- The small GTPase Rap1 is a modulator of Hedgehog signaling.Developmental biology · 2016Article
- Rap1 GTPase is required for mouse lens epithelial maintenance and morphogenesis.Developmental biology · 2015Article
- Substrate trapping proteomics reveals targets of the βTrCP2/FBXW11 ubiquitin ligase.Molecular and cellular biology · 2015Article
- Rapgef2 connects GPCR-mediated cAMP signals to ERK activation in neuronal and endocrine cells.Science signaling · 2013Article
- Distinct Rap1 activity states control the extent of epithelial invagination via α-catenin.Developmental cell · 2013Article
- New spin on an old transition: epithelial parallels in neuronal adhesion control.Trends in neurosciences · 2013Review
- Rap1 GTPase activation and barrier enhancement in rpe inhibits choroidal neovascularization in vivo.PloS one · 2013Article
- Article
- Myosin II isoforms identify distinct functional modules that support integrity of the epithelial zonula adherens.Nature cell biology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Epithelial morphogenesis is characterized by an exquisite control of cell shape and position. Progression through dorsal closure in Drosophila gastrulation depends on the ability of Rap1 GTPase to signal through the adherens junctional multidomain protein Canoe. Here, we provide genetic evidence that epithelial Rap activation and Canoe effector usage are conferred by the Drosophila PDZ-GEF (dPDZ-GEF) exchange factor. We demonstrate that dPDZ-GEF/Rap/Canoe signaling modulates cell shape and apicolateral cell constriction in embryonic and wing disc epithelia. In dPDZ-GEF mutant embryos with strong dorsal closure defects, cells in the lateral ectoderm fail to properly elongate. Postembryonic dPDZ-GEF mutant cells generated in mosaic tissue display a striking extension of lateral cell perimeters in the proximity of junctional complexes, suggesting a loss of normal cell contractility. Furthermore, our data indicate that dPDZ-GEF signaling is linked to myosin II function. Both dPDZ-GEF and cno show strong genetic interactions with the myosin II-encoding gene, and myosin II distribution is severely perturbed in epithelia of both mutants. These findings provide the first insight into the molecular machinery targeted by Rap signaling to modulate epithelial plasticity. We propose that dPDZ-GEF-dependent signaling functions as a rheostat linking Rap activity to the regulation of cell shape in epithelial morphogenesis at different developmental stages.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.