ArticleMolecular and cellular biology2007
USF1 recruits histone modification complexes and is critical for maintenance of a chromatin barrier.
Article in Molecular and cellular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
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77 citing papers in PubMed, 121 citations in OpenAlex.
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- The dynamic broad epigenetic (H3K4me3, H3K27ac) domain as a mark of essential genes.Clinical epigenetics · 2021Review
- Parental nucleosome segregation and the inheritance of cellular identity.Nature reviews. Genetics · 2021Review
- Regulatory sharing between estrogen receptor α bound enhancers.Nucleic acids research · 2020Article
- Role of Isocitrate Dehydrogenase 2 on DNA Hydroxymethylation in Human Airway Smooth Muscle Cells.American journal of respiratory cell and molecular biology · 2020Article
- Sustained transgene expression from sleeping beauty DNA transposons containing a core fragment of the HNRPA2B1-CBX3 ubiquitous chromatin opening element (UCOE).BMC biotechnology · 2019Article
- Epigenomic analysis reveals DNA motifs regulating histone modifications in human and mouse.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Neuronal Expression of Opioid Gene is Controlled by Dual Epigenetic and Transcriptional Mechanism in Human Brain.Cerebral cortex (New York, N.Y. : 1991) · 2018Article
- Article
- Hypermethylated LTR retrotransposon exhibits enhancer activity.Epigenetics · 2017Article
- The pregnane X receptor down-regulates organic cation transporter 1 (SLC22A1) in human hepatocytes by competing for ("squelching") SRC-1 coactivator.British journal of pharmacology · 2016Article
- Vascular smooth muscle LRP6 limits arteriosclerotic calcification in diabetic LDLR-/- mice by restraining noncanonical Wnt signals.Circulation research · 2015Article
- The human ankyrin 1 promoter insulator sustains gene expression in a β-globin lentiviral vector in hematopoietic stem cells.Molecular therapy. Methods & clinical development · 2015Article
- Genome-wide predictors of NF-κB recruitment and transcriptional activity.BioData mining · 2015Article
- Histone demethylase LSD1-mediated repression of GATA-2 is critical for erythroid differentiation.Drug design, development and therapy · 2015Article
17 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
Abstract
The insulator element at the 5' end of the chicken beta-globin locus acts as a barrier, protecting transgenes against silencing effects of adjacent heterochromatin. We showed earlier that the transcription factor USF1 binds within the insulator and that this site is important for generating in adjacent nucleosomes histone modifications associated with active chromatin and, by inference, with barrier function. To understand the mechanism of USF1 action, we have characterized USF1-containing complexes. USF1 interacts directly with the histone H4R3-specific methyltransferase PRMT1. USF1, PRMT1, and the histone acetyltransferases (HATs) PCAF and SRC-1 form a complex with both H4R3 histone methyltransferase and HAT activities. Small interfering RNA downregulation of USF1 results in localized loss of H4R3 methylation, and other histone modifications associated with euchromatin, at the insulator. A dominant negative peptide that interferes with USF1 binding to DNA causes silencing of an insulated reporter construct, indicating abolition of barrier function. These results show that USF1 plays a direct role in maintaining the barrier, supporting a model in which the insulator works as a barrier by maintaining a local environment of active chromatin.
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