ArticleMolecular biotechnology2007
MdACO expression during abscission: the use of 33P labeled primers in transcript quantitation.
Article in Molecular biotechnology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 13 citations in OpenAlex.
- Phytohormonal dynamics in the abscission zone of Korla fragrant pear during calyx abscission: a visual study.Frontiers in plant science · 2024Article
- Ethylene-regulated immature fruit abscission is associated with higher expression ofRoyal Society open science · 2021Article
- Transcriptomic signatures in seeds of apple (Malus domestica L. Borkh) during fruitlet abscission.PloS one · 2015Article
- Identification of genes in the phenylalanine metabolic pathway by ectopic expression of a MYB transcription factor in tomato fruit.The Plant cell · 2011Article
- Dominance induction of fruitlet shedding in Malus x domestica (L. Borkh): molecular changes associated with polar auxin transport.BMC plant biology · 2009Article
- Identification of Solanum habrochaites loci that quantitatively influence tomato fruit ripening-associated ethylene emissions.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2009Article
- Isolation and characterization of genes associated with shade-induced apple abscission.Molecular genetics and genomics : MGG · 2008Article
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The last step of ethylene biosynthesis in apple is catalyzed by ACO (1-aminocyclopropane 1-carboxylic acid oxidase) encoded by MdACO1 and MdACO2. Both genes were expressed during early fruit development although at different level. Later on, the ACO transcript accumulation in persisting fruitlets decreased whereas in fruitlets undergoing abscission MdACO2 transcripts remained constant and those of MdACO1dramatically increased. The expression of MdACO was assessed by a particular type of semi-quantitative PCR involving 33P labeled primers and the following electrophoresis in polyacrylamide gel. The use of either the reverse primer or the anchored oligo dT13 produced clear and elegant results. Nevertheless, the initial quantity of labeled primer appeared to be a crucial factor for obtaining a wide range titration curve.
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