ArticleApplied and environmental microbiology2007
Detection and quantification of Geobacter lovleyi strain SZ: implications for bioremediation at tetrachloroethene- and uranium-impacted sites.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 54 citations in OpenAlex.
- Environmental considerations and bioremediation applications of the metal-, nitrate-, and organohalide-respiring bacteriumApplied and environmental microbiology · 2026Review
- Generation of zero-valent sulfur from dissimilatory sulfate reduction in sulfate-reducing microorganisms.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Organohalide respiration potential in marine sediments from Aarhus Bay.FEMS microbiology ecology · 2022Article
- 2,4-Dichlorophenoxyacetic acid degradation in methanogenic mixed cultures obtained from Brazilian Amazonian soil samples.Biodegradation · 2021Article
- Reductive dechlorination of 1,2-dichloroethane in the presence of chloroethenes and 1,2-dichloropropane as co-contaminants.Applied microbiology and biotechnology · 2019Article
- Efficient Bioelectrochemical Conversion of Industrial Wastewater by Specific Strain Isolation and Community Adaptation.Frontiers in bioengineering and biotechnology · 2019Article
- Presence, Diversity, and Enrichment of Respiratory Reductive Dehalogenase and Non-respiratory Hydrolytic and Oxidative Dehalogenase Genes in Terrestrial Environments.Frontiers in microbiology · 2019Article
- Natural Attenuation in Streambed Sediment Receiving Chlorinated Solvents from Underlying Fracture Networks.Environmental science & technology · 2017Article
- Recent Origin of the Methacrylate Redox System in Geobacter sulfurreducens AM-1 through Horizontal Gene Transfer.PloS one · 2015Article
- Cell-secreted flavins bound to membrane cytochromes dictate electron transfer reactions to surfaces with diverse charge and pH.Scientific reports · 2014Article
- Molecular analysis of the in situ growth rates of subsurface Geobacter species.Applied and environmental microbiology · 2013Article
- Genomic determinants of organohalide-respiration in Geobacter lovleyi, an unusual member of the Geobacteraceae.BMC genomics · 2012Article
- Molecular techniques in the biotechnological fight against halogenated compounds in anoxic environments.Microbial biotechnology · 2012Review
- Dynamics of microbial community composition and function during in situ bioremediation of a uranium-contaminated aquifer.Applied and environmental microbiology · 2011Article
- Microbial community changes in response to ethanol or methanol amendments for U(VI) reduction.Applied and environmental microbiology · 2010Article
- Unique ecophysiology among U(VI)-reducing bacteria as revealed by evaluation of oxygen metabolism in Anaeromyxobacter dehalogenans strain 2CP-C.Applied and environmental microbiology · 2010Article
- Diversity and distribution of anaeromyxobacter strains in a uranium-contaminated subsurface environment with a nonuniform groundwater flow.Applied and environmental microbiology · 2009Article
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Geobacter lovleyi strain SZ reduces hexavalent uranium, U(VI), to U(IV) and is the first member of the metal-reducing Geobacter group capable of using tetrachloroethene (PCE) as a growth-supporting electron acceptor. Direct and nested PCR with specific 16S rRNA gene-targeted primer pairs distinguished strain SZ from other known chlorinated ethene-dechlorinating bacteria and closely related Geobacter isolates, including its closest cultured relative, G. thiogenes. Detection limits for direct and nested PCR were approximately 1 x 10(6) and 1 x 10(4) 16S rRNA gene copies per mul of template DNA, respectively. A quantitative real-time PCR (qPCR) approach increased the sensitivity to as few as 30 16S rRNA gene copies per mul of template DNA but was less specific. Melting curve analysis and comparison of the shapes of amplification plots identified false-positive signals and distinguished strain SZ from G. thiogenes when analyzed separately. These indicators were less reliable when target (strain SZ) DNA and nontarget (G. thiogenes) DNA with high sequence similarity were mixed, indicating that the development of qPCR protocols should not only evaluate specificity but also explore the effects of nontarget DNA on the accuracy of quantification. Application of specific tools detected strain SZ-like amplicons in PCE-dechlorinating consortia, including the bioaugmentation consortium KB-1, and two chlorinated ethene-impacted groundwater samples. Strain SZ-like amplicons were also detected in 13 of 22 groundwater samples following biostimulation at the uranium- and chlorinated solvent-contaminated Integrated Field-Scale Subsurface Research Challenge (IFC) site in Oak Ridge, TN. The numbers of strain SZ-like cells increased from below detection to 2.3 x 10(7) +/- 0.1 x 10(7) per liter groundwater, suggesting that strain SZ-like organisms contribute to contaminant transformation. The G. lovleyi strain SZ-specific tools will be useful for monitoring bioremediation efforts at uranium- and/or chlorinated solvent-impacted sites such as the Oak Ridge IFC site.
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