Evidence map›Paper›PMID 17827308›Full record

ArticleApplied and environmental microbiology2007

Methanobrevibacter ruminantium as an indicator of domesticated-ruminant fecal pollution in surface waters.

Jennifer A Ufnar, Shiao Y Wang, David F Ufnar, R D Ellender

Abstract read
In one paragraph

Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Metagenomic Comparison of Gut Microbes ofAnimals : an open access journal from MDPI · 2025
    Article
  2. Article
  3. Article
  4. Review
  5. Review
  6. Enterococci in the environment.Microbiology and molecular biology reviews : MMBR · 2012
    Review
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Jennifer A UfnarDepartment of Biological Sciences, The University of Southern Mississippi, Hattiesburg, Mississippi 39406, USA. jennifer.a.ufnar@vanderbilt.edu
Shiao Y Wang
David F Ufnar
R D Ellender

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A PCR-based assay (Mrnif) targeting the nifH gene of Methanobrevibacter ruminantium was developed to detect fecal pollution from domesticated ruminants in environmental water samples. The assay produced the expected amplification product only when the reaction mixture contained DNA extracted from M. ruminantium culture, bovine (80%), sheep (100%), and goat (75%) feces, and water samples from a bovine waste lagoon (100%) and a creek contaminated with bovine lagoon waste (100%). The assay appears to be specific and sensitive and can distinguish between domesticated- and nondomesticated-ruminant fecal pollution in environmental samples.

Indexed as

Water MicrobiologyAnimalsAnimals, DomesticCattleEnvironmental MonitoringFecesFresh WaterMethanobrevibacterMolecular Sequence DataOxidoreductasesPolymerase Chain ReactionRuminantsSensitivity and SpecificityWater Pollutionnitrogenase reductaseOxidoreductases

Identifiers

PMID17827308
PMCPMC2074949

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.