ArticleBiophysical journal2008
Directly monitoring individual retrovirus budding events using atomic force microscopy.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed.
- 3D localization of retrovirus assembly in the presence of structured background with deep learning.Biophysical journal · 2025Article
- From viral assembly to host interaction: AFM's contributions to virology.Journal of virology · 2025Review
- An accurate probabilistic step finder for time-series analysis.Biophysical journal · 2024Article
- An accurate probabilistic step finder for time-series analysis.bioRxiv : the preprint server for biology · 2023Article
- High-speed AFM reveals the dynamics of virus budding.Biophysical journal · 2022Article
- Analysis of individual HIV-1 budding event using fast AFM reveals a multiplexed role for VPS4.Biophysical journal · 2022Article
- Review
- Review
- Towards a Quantitative Single Particle Characterization by Super Resolution Microscopy: From Virus Structures to Antivirals Design.Frontiers in bioengineering and biotechnology · 2021Review
- Single-particle virology.Biophysical reviews · 2020Review
- Review
- First passage events in biological systems with non-exponential inter-event times.Scientific reports · 2018Article
- Algal Viruses: The (Atomic) Shape of Things to Come.Viruses · 2018Review
- A novel minimal in vitro system for analyzing HIV-1 Gag-mediated budding.Journal of biological physics · 2015Article
- Photothermal imaging and measurement of protein shell stoichiometry of single HIV-1 Gag virus-like nanoparticles.ACS nano · 2011Article
- Visualizing HIV-1 assembly.Journal of molecular biology · 2011Review
- Sampling protein form and function with the atomic force microscope.Molecular & cellular proteomics : MCP · 2010Review
- Retroviral assembly and budding occur through an actin-driven mechanism.Biophysical journal · 2009Article
- Dynamics of HIV-1 assembly and release.PLoS pathogens · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Retrovirus budding is a key step in the virus replication cycle. Nonetheless, very little is known about the underlying mechanism of budding, primarily due to technical limitations preventing visualization of bud formation in real time. Methods capable of monitoring budding dynamics suffer from insufficient resolution, whereas other methods, such as electron microscopy, do not have the ability to operate under physiological conditions. Here we applied atomic force microscopy to real-time visualization of individual Moloney murine leukemia virus budding events. By using a single-particle analysis approach, we were able to observe distinct patterns in budding that otherwise remain transparent. We find that bud formation follows at least two kinetically distinct pathways. The majority of virions (74%) are produced in a slow process (>45 min), and the remaining particles (26%) assemble via a fast process (<25 min). Interestingly, repetitive budding from the same site was seen to occur in only two locations. This finding challenges the hypothesis that viral budding occurs from distinct sites and suggests that budding is not restricted laterally. In this study, we established a method to monitor the fine dynamics of the virus budding process. Using this single-particle analysis to study mutated viruses will enable us to gain additional insight into the mechanisms of viral budding.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.