ArticleBiophysical journal2008
Supercritical angle fluorescence correlation spectroscopy.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 52 citations in OpenAlex.
- Combinatorial sample- and back-focal-plane imaging. Pt. I: Instrument and acquisition parameters affecting BFP images and their analysis.Biophysical journal · 2025Article
- Direct supercritical angle localization microscopy for nanometer 3D superresolution.Nature communications · 2021Article
- Supercritical Angle Fluorescence Microscopy and Spectroscopy.Biophysical journal · 2020Review
- Time-Resolved Laurdan Fluorescence Reveals Insights into Membrane Viscosity and Hydration Levels.Biophysical journal · 2018Article
- Simultaneous Surface-Near and Solution Fluorescence Correlation Spectroscopy.Journal of fluorescence · 2016Article
- Measuring membrane association and protein diffusion within membranes with supercritical angle fluorescence microscopy.Biomedical optics express · 2016Article
- Recent developments in fluorescence correlation spectroscopy for diffusion measurements in planar lipid membranes.International journal of molecular sciences · 2010Review
- Photonic methods to enhance fluorescence correlation spectroscopy and single molecule fluorescence detection.International journal of molecular sciences · 2010Review
- Total internal reflection with fluorescence correlation spectroscopy: Applications to substrate-supported planar membranes.Journal of structural biology · 2009Article
- Surface modified single molecules free-diffusion evidenced by fluorescence correlation spectroscopy.Journal of fluorescence · 2008Article
- Total internal reflection fluorescence correlation spectroscopy: effects of lateral diffusion and surface-generated fluorescence.Biophysical journal · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We explore the potential of a supercritical angle (SA) objective for fluorescence correlation spectroscopy (FCS). This novel microscope objective combines tight focusing by an aspheric lens with strong axial confinement of supercritical angle fluorescence collection by a parabolic mirror lens, resulting in a small detection volume. The tiny axial extent of the detection volume features an excellent surface sensitivity, as is demonstrated by diffusion measurements in model membranes with an excess of free dye in solution. All SA-FCS measurements are directly compared to standard confocal FCS, demonstrating a clear advantage of SA-FCS, especially for diffusion measurements in membranes. We present an extensive theoretical framework that allows for accurate and quantitative evaluation of the SA-FCS correlation curves.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.