ArticleJournal of molecular biology2007
The rough energy landscape of superfolder GFP is linked to the chromophore.
Article in Journal of molecular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- Live-cell co-translational folding tracking reveals bidirectional coupling between translation and folding.bioRxiv : the preprint server for biology · 2026Article
- ATP binding to lysozyme and superfolder GFP amyloid fibrils induces aggregate remodeling and attenuates their cytotoxicity.Cell death discovery · 2026Article
- Selecting aminoacyl-tRNA synthetase/tRNA pairs for efficient genetic encoding of noncanonical amino acids into proteins.Nature protocols · 2026Review
- Biochemical and functional characterization ofFrontiers in microbiology · 2022Article
- Characterization of Fluorescent Proteins with Intramolecular Photostabilization*.Chembiochem : a European journal of chemical biology · 2021Article
- β-Barrels and Amyloids: Structural Transitions, Biological Functions, and Pathogenesis.International journal of molecular sciences · 2021Review
- Amino acid residue at the 165th position tunes EYFP chromophore maturation. A structure-based design.Computational and structural biotechnology journal · 2021Article
- Live-cell imaging of single mRNA dynamics using split superfolder green fluorescent proteins with minimal background.RNA (New York, N.Y.) · 2020Article
- Association of Fluorescent Protein Pairs and Its Significant Impact on Fluorescence and Energy Transfer.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
- Seeing the long tail: A novel green fluorescent protein, SiriusGFP, for ultra long timelapse imaging.Journal of neuroscience methods · 2019Article
- In vivo selection of sfGFP variants with improved and reliable functionality in industrially important thermophilic bacteria.Biotechnology for biofuels · 2018Article
- Characterization of Annexin V Fusion with the Superfolder GFP in Liposomes Binding and Apoptosis Detection.Frontiers in physiology · 2017Article
- Peculiarities of the Super-Folder GFP Folding in a Crowded Milieu.International journal of molecular sciences · 2016Article
- Article
- Untangling the Influence of a Protein Knot on Folding.Biophysical journal · 2016Article
- Heterogeneous side chain conformation highlights a network of interactions implicated in hysteresis of the knotted protein, minimal tied trefoil.Journal of physics. Condensed matter : an Institute of Physics journal · 2015Article
- Structural plasticity of green fluorescent protein to amino acid deletions and fluorescence rescue by folding-enhancing mutations.BMC biochemistry · 2015Article
- Efficient expression of codon-adapted affinity tagged super folder green fluorescent protein for synchronous protein localization and affinity purification studies in Tetrahymena thermophila.BMC biotechnology · 2015Article
- Preparation of Specific Polyclonal Antibody Against the Recombinant Mutacin Produced by sfGFP Fusion Protein Technology.The open microbiology journal · 2015Article
- Characterization of camel nanobodies specific for superfolder GFP fusion proteins.Molecular biology reports · 2014Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Many green fluorescent protein (GFP) variants have been developed for use as fluorescent tags, and recently a superfolder GFP (sfGFP) has been developed as a robust folding reporter. This new variant shows increased stability and improved folding kinetics, as well as 100% recovery of native protein after denaturation. Here, we characterize sfGFP, and find that this variant exhibits hysteresis as unfolding and refolding equilibrium titration curves are non-coincident even after equilibration for more than eight half-lives as estimated from kinetic unfolding and refolding studies. This hysteresis is attributed to trapping in a native-like intermediate state. Mutational studies directed towards inhibiting chromophore formation indicate that the novel backbone cyclization is responsible for the hysteresis observed in equilibrium titrations of sfGFP. Slow equilibration and the presence of intermediates imply a rough landscape. However, de novo folding in the absence of the chromophore is dominated by a smoother energy landscape than that sampled during unfolding and refolding of the post-translationally modified polypeptide.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.