ArticleProceedings of the National Academy of Sciences of the United States of America2007
Real-time observations of single bacteriophage lambda DNA ejections in vitro.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed.
- Viral Genome Delivery Across Bacterial Cell Surfaces.Annual review of microbiology · 2024Review
- Temperature-dependent ejection evolution arising from active and passive effects in DNA viruses.Biophysical journal · 2024Article
- Article
- Theoretical Studies on Assembly, Physical Stability, and Dynamics of Viruses.Sub-cellular biochemistry · 2024Review
- Role of DNA-DNA sliding friction and nonequilibrium dynamics in viral genome ejection and packaging.Nucleic acids research · 2023Article
- AT-specific DNA visualization revisits the directionality of bacteriophage λ DNA ejection.Nucleic acids research · 2023Article
- Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies.Journal of visualized experiments : JoVE · 2022Article
- Imaging the Infection Cycle of T7 at the Single Virion Level.International journal of molecular sciences · 2022Article
- Single-particle virology.Biophysical reviews · 2020Review
- C22 podovirus infectivity is associated with intermediate stiffness.Scientific reports · 2020Article
- Additive manufacturing of laminar flow cells for single-molecule experiments.Scientific reports · 2019Article
- A packing for A-form DNA in an icosahedral virus.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Time-resolved DNA release from an O-antigen-specificThe Journal of biological chemistry · 2019Article
- The phage L capsid decoration protein has a novel OB-fold and an unusual capsid binding strategy.eLife · 2019Article
- High-resolution studies of lysis-lysogeny decision-making in bacteriophage lambda.The Journal of biological chemistry · 2019Review
- Article
- Late-Arriving Signals Contribute Less to Cell-Fate Decisions.Biophysical journal · 2017Article
- Phage-like packing structures with mean field sequence dependence.Journal of computational chemistry · 2017Article
- Translocation of a granular chain in a horizontally vibrated saw-tooth channel.The European physical journal. E, Soft matter · 2016Article
- The structure and intermolecular forces of DNA condensates.Nucleic acids research · 2016Article
Corrections and comments
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Authors and funding
4 authors.
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Abstract
The physical, chemical, and structural features of bacteriophage genome release have been the subject of much recent attention. Many theoretical and experimental studies have centered on the internal forces driving the ejection process. Recently, Mangenot et al. [Mangenot S, Hochrein M, Rädler J, Letellier L (2005) Curr Biol 15:430-435.] reported fluorescence microscopy of phage T5 ejections, which proceeded stepwise between DNA nicks, reaching a translocation speed of 75 kbp/s or higher. It is still unknown how high the speed actually is. This paper reports real-time measurements of ejection from phage lambda, revealing how the speed depends on key physical parameters such as genome length and ionic state of the buffer. Except for a pause before DNA is finally released, the entire 48.5-kbp genome is translocated in approximately 1.5 s without interruption, reaching a speed of 60 kbp/s. The process gives insights particularly into the effects of two parameters: a shorter genome length results in lower speed but a shorter total time, and the presence of divalent magnesium ions (replacing sodium) reduces the pressure, increasing ejection time to 8-11 s. Pressure caused by DNA-DNA interactions within the head affects the initiation of ejection, but the close packing is also the dominant source of friction: more tightly packed phages initiate ejection earlier, but with a lower initial speed. The details of ejection revealed in this study are probably generic features of DNA translocation in bacteriophages and have implications for the dynamics of DNA in other biological systems.
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