ArticleJournal of cellular physiology2008
Differential signaling mechanisms of HNP-induced IL-8 production in human lung epithelial cells and monocytes.
Article in Journal of cellular physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 35 citations in OpenAlex.
- HNP-1: From Structure to Application Thanks to Multifaceted Functions.Microorganisms · 2025Review
- Alpha-defensins inhibit ERK/STAT3 signaling during monocyte-macrophage differentiation and impede macrophage function.Respiratory research · 2023Article
- Reversal of Azole Resistance inBiomedicines · 2023Article
- Physiologic roles of P2 receptors in leukocytes.Journal of leukocyte biology · 2022Review
- Elevated Monocytic Interleukin-8 Expression under Intermittent Hypoxia Condition and in Obstructive Sleep Apnea Patients.International journal of molecular sciences · 2021Article
- Host Defense Peptides as Templates for Antifungal Drug Development.Journal of fungi (Basel, Switzerland) · 2020Review
- Multifaceted immune functions of human defensins and underlying mechanisms.Seminars in cell & developmental biology · 2019Review
- Dual effects of human neutrophil peptides in a mouse model of pneumonia and ventilator-induced lung injury.Respiratory research · 2018Article
- Macrocyclic θ-defensins suppress tumor necrosis factor-α (TNF-α) shedding by inhibition of TNF-α-converting enzyme.The Journal of biological chemistry · 2018Article
- Human Neutrophil Defensin-1, -3, and -4 Are Elevated in Nasal Aspirates from Children with Naturally Occurring Adenovirus Infection.Canadian respiratory journal · 2018Article
- Innate Immunity to Mucosal Candida Infections.Journal of fungi (Basel, Switzerland) · 2017Review
- Rhesus macaque θ-defensin RTD-1 inhibits proinflammatory cytokine secretion and gene expression by inhibiting the activation of NF-κB and MAPK pathways.Journal of leukocyte biology · 2015Article
- Rhesus macaque theta defensins suppress inflammatory cytokines and enhance survival in mouse models of bacteremic sepsis.PloS one · 2012Article
- Circadian rhythms: from basic mechanisms to the intensive care unit.Critical care medicine · 2012Review
- High fidelity processing and activation of the human α-defensin HNP1 precursor by neutrophil elastase and proteinase 3.PloS one · 2012Article
- Neutrophil alpha-defensins cause lung injury by disrupting the capillary-epithelial barrier.American journal of respiratory and critical care medicine · 2010Article
- Alpha-defensins increase lung fibroblast proliferation and collagen synthesis via the beta-catenin signaling pathway.The FEBS journal · 2009Article
- Human neutrophil peptides and phagocytic deficiency in bronchiectatic lungs.American journal of respiratory and critical care medicine · 2009Article
- The role of antimicrobial peptides at the ocular surface.Ophthalmic research · 2009Review
- Human neutrophil peptides: a novel potential mediator of inflammatory cardiovascular diseases.American journal of physiology. Heart and circulatory physiology · 2008Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Human neutrophil peptides (HNP) kill microorganisms but also modulate immune responses through upregulation of the chemokine IL-8 by activation of the nucleotide P2Y(6) receptor. However, the intracellular signaling mechanisms remain yet to be determined. Human lung epithelial cells (A549) and monocytes (U937) were stimulated with HNP in the absence and presence of the specific kinase inhibitors for Src, extracellular signal-regulated kinase-1 and -2 (ERK1/2), p38 mitogen-activated protein kinase (MAPK), c-Jun-N-terminal kinases (JNK), and Akt. HNP induced a rapid phosphorylation of the kinases in both cell types associated with a dose-dependent, selective production of IL-8 among 10 cytokines assayed. The HNP-induced IL-8 production was blocked by the Src tyrosine kinase inhibitor PP2, MEK1/2 inhibitor U0126, and the phosphatidylinositol 3 kinase (PI3K) inhibitor LY294002, but not by the JNK inhibitor SP600125 in both cell types. Treatment with the p38 inhibitor SB203580 attenuated the HNP-induced IL-8 production only in monocytes. Blockade of Src kinase blunted HNP-induced phosphorylation of the ERK1/2 and Akt but not p38 in monocytes. In contrast, Src inhibition had no effect on phosphorylation of the other kinases in the lung epithelial cells. We conclude that the activation of ERK1/2 and PI3K/Akt pathways is required for HNP-induced IL-8 release which occurs in a Src-independent manner in lung epithelial cells, while is Src-dependent in monocytes.
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