ArticleSmall (Weinheim an der Bergstrasse, Germany)2007
Novel biocatalysts based on S-layer self-assembly of Geobacillus stearothermophilus NRS 2004/3a: a nanobiotechnological approach.
Article in Small (Weinheim an der Bergstrasse, Germany), 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 54 citations in OpenAlex.
- Supramolecular protein assembly in cell-free protein synthesis system.Bioresources and bioprocessing · 2022Article
- Protein Assemblies: Nature-Inspired and Designed Nanostructures.Biomacromolecules · 2019Review
- Slp-coated liposomes for drug delivery and biomedical applications: potential and challenges.International journal of nanomedicine · 2019Review
- Structural basis of cell wall anchoring by SLH domains in Paenibacillus alvei.Nature communications · 2018Article
- Relevance of glycosylation of S-layer proteins for cell surface properties.Acta biomaterialia · 2015Article
- Emulsomes meet S-layer proteins: an emerging targeted drug delivery system.Current pharmaceutical biotechnology · 2015Review
- S-layers: principles and applications.FEMS microbiology reviews · 2014Article
- Protein bodies in nature and biotechnology.Molecular biotechnology · 2013Article
- Bacterial cell-envelope glycoconjugates.Advances in carbohydrate chemistry and biochemistry · 2013Review
- A fusion tag to fold on: the S-layer protein SgsE confers improved folding kinetics to translationally fused enhanced green fluorescent protein.Journal of microbiology and biotechnology · 2012Article
- S-layer fusion proteins--construction principles and applications.Current opinion in biotechnology · 2011Review
- Recombinant glycans on an S-layer self-assembly protein: a new dimension for nanopatterned biomaterials.Small (Weinheim an der Bergstrasse, Germany) · 2008Article
- S-layer nanoglycobiology of bacteria.Carbohydrate research · 2008Review
- Molecular basis of S-layer glycoprotein glycan biosynthesis in Geobacillus stearothermophilus.The Journal of biological chemistry · 2008Article
- Exploitation of the S-layer self-assembly system for site directed immobilization of enzymes demonstrated for an extremophilic laminarinase from Pyrococcus furiosus.Journal of biotechnology · 2008Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
The crystalline cell-surface (S) layer sgsE of Geobacillus stearothermophilus NRS 2004/3a represents a natural protein self-assembly system with nanometer-scale periodicity that is evaluated as a combined carrier/patterning element for the conception of novel types of biocatalyst aiming at the controllable display of biocatalytic epitopes, storage stability, and reuse. The glucose-1-phosphate thymidylyltransferase RmlA is used as a model enzyme and chimeric proteins are constructed by translational fusion of rmlA to the C-terminus of truncated forms of sgsE (rSgsE (131-903), rSgsE(331-903)) and used for the construction of three principal types of biocatalysts: soluble (monomeric), self-assembled in aqueous solution, and recrystallized on negatively charged liposomes. Enzyme activity of the biocatalysts reaches up to 100 % compared to sole RmlA cloned from the same bacterium. The S-layer portion of the biocatalysts confers significantly improved shelf life to the fused enzyme without loss of activity over more than three months, and also enables biocatalyst recycling. These nanopatterned composites may open up new functional concepts for biocatalytic applications in nanobiotechnology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.