ArticleJournal of molecular histology2008
In vitro wounding: effects of hypoxia and transforming growth factor beta1 on proliferation, migration and myofibroblastic differentiation in an endothelial cell-fibroblast co-culture model.
Article in Journal of molecular histology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed.
- Comparative Late Effects of Hemostatic Biomaterials on Wound Healing at 14 and 30 Days: An In Vivo Animal Study.Journal of functional biomaterials · 2026Article
- Platelet derived growth factor-AB modulates post-infarct myocardium leading to extended improvement in cardiac function.NPJ Regenerative medicine · 2025Article
- Article
- Effect of Gelatin-Based Hemostats on Fibroblasts and Relevant Growth Factors in Wound Healing.Gels (Basel, Switzerland) · 2023Article
- Exosomal miR-767 from senescent endothelial-derived accelerating skin fibroblasts aging via inhibiting TAB1.Journal of molecular histology · 2023Article
- Reconstituting the dynamics of endothelial cells and fibroblasts in wound closure.APL bioengineering · 2021Article
- Synthetic Calcium Phosphate Ceramics as a Potential Treatment for Bisphosphonate-Related Osteonecrosis of the Jaw.Materials (Basel, Switzerland) · 2019Article
- FK506-binding protein 10 (FKBP10) regulates lung fibroblast migration via collagen VI synthesis.Respiratory research · 2018Article
- Fluorinated Methacrylamide Chitosan Hydrogels Enhance Cellular Wound Healing Processes.Annals of biomedical engineering · 2017Article
- Skin Wound Healing Potential and Mechanisms of the Hydroalcoholic Extract of Leaves and Oleoresin ofEvidence-based complementary and alternative medicine : eCAM · 2017Article
- Impact of single-nucleotide polymorphisms on radiation pneumonitis in cancer patients.Molecular and clinical oncology · 2016Article
- PDGFRα plays a crucial role in connective tissue remodeling.Scientific reports · 2015Article
- On-chip assay of the effect of topographical microenvironment on cell growth and cell-cell interactions during wound healing.Biomicrofluidics · 2015Article
- Effect of growth factors on antimicrobial peptides and pro-inflammatory mediators during wound healing.Clinical oral investigations · 2015Article
- ZNF580 mediates eNOS expression and endothelial cell migration/proliferation via the TGF-β1/ALK5/Smad2 pathway.Molecular and cellular biochemistry · 2014Article
- Hypoxia preconditioned mesenchymal stem cells prevent cardiac fibroblast activation and collagen production via leptin.PloS one · 2014Article
- Soft tissue fibroblasts from well healing and chronic human wounds show different rates of myofibroblasts in vitro.Molecular biology reports · 2013Article
- Hypercapnia accelerates wound healing in endothelial cell monolayers exposed to hypoxia.The open respiratory medicine journal · 2013Article
- Low oxygen tension positively influences cardiomyocyte progenitor cell function.Journal of cellular and molecular medicine · 2011Article
- Erythropoietin ameliorates the reduced migration of human fibroblasts during in vitro hypoxia.Journal of physiology and biochemistry · 2011Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The adequate reconstitution of human soft tissue wounds requires the coordinated interaction of endothelial cells and fibroblasts during the proliferation phase of healing. Endothelial cells assure neoangiogenesis, fibroblasts fill the defect and provide extracellular matrix proteins, and myofibroblasts are believed to support the reconstitution of microvessels. In the present study, we combined in vitro-wound size measurement and multicolour immunocytochemical staining of co-cultured human dermal microvascular endothelial cells and normal human dermal fibroblasts, recently introduced as co-culture scratch-wound migration assay. Applying antibodies for alpha-smooth-muscle actin, von Willebrand factor, extra domain A fibronectin and endothelin-1, we were able to monitor proliferation, migration and the differentiation process from fibroblasts to myofibroblasts as a response to hypoxia. Furthermore, we verified, whether transforming growth factor beta1 (TGFbeta1) and endothelin-1 are able to mediate this response. We show, that proliferation and migration of endothelial cells and fibroblasts decreased under hypoxia. The additional administration of TGFbeta1 did not significantly attenuate this decrease. Solely the myofibroblast population in co-culture adapted well to hypoxia, when cultures were supplemented with TGFbeta1. Considerating the data concerning TGFbeta1 and endothelin-1, we propose a model explaining the cellular interaction during early and late proliferation phase of human wound healing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.