ArticlePloS one2007
Deletion of the N-terminus of SF2/ASF permits RS-domain-independent pre-mRNA splicing.
Article in PloS one, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
- Mechanism of alternative splicing of yeast HEH1 through competing 5' splice sites.Nature communications · 2025Article
- Unearthing a novel function of SRSF1 in binding and unfolding of RNA G-quadruplexes.Nucleic acids research · 2024Article
- Unearthing SRSF1's Novel Function in Binding and Unfolding of RNA G-Quadruplexes.bioRxiv : the preprint server for biology · 2023Article
- Towards understandings of serine/arginine-rich splicing factors.Acta pharmaceutica Sinica. B · 2023Review
- Article
- Cooperative engagement and subsequent selective displacement of SR proteins define the pre-mRNA 3D structural scaffold for early spliceosome assembly.Nucleic acids research · 2022Article
- CircRPAP2 regulates the alternative splicing of PTK2 by binding to SRSF1 in breast cancer.Cell death discovery · 2022Article
- Exon-independent recruitment of SRSF1 is mediated by U1 snRNP stem-loop 3.The EMBO journal · 2022Article
- Discovery of a pre-mRNA structural scaffold as a contributor to the mammalian splicing code.Nucleic acids research · 2021Article
- Structure of SRSF1 RRM1 bound to RNA reveals an unexpected bimodal mode of interaction and explains its involvement in SMN1 exon7 splicing.Nature communications · 2021Article
- Emerging functions of SRSF1, splicing factor and oncoprotein, in RNA metabolism and cancer.Molecular cancer research : MCR · 2014Review
- Downregulation of splicing factor SRSF3 induces p53β, an alternatively spliced isoform of p53 that promotes cellular senescence.Oncogene · 2013Article
- Pick one, but be quick: 5' splice sites and the problems of too many choices.Genes & development · 2013Review
- Mechanisms of activation and repression by the alternative splicing factors RBFOX1/2.RNA (New York, N.Y.) · 2012Article
- Mutation of a U2 snRNA gene causes global disruption of alternative splicing and neurodegeneration.Cell · 2012Article
- The splicing factor SRSF1 regulates apoptosis and proliferation to promote mammary epithelial cell transformation.Nature structural & molecular biology · 2012Article
- Molecular design of a splicing switch responsive to the RNA binding protein Tra2β.Nucleic acids research · 2011Article
- Doublesex: a conserved downstream gene controlled by diverse upstream regulators.Journal of genetics · 2010Review
- Cooperative-binding and splicing-repressive properties of hnRNP A1.Molecular and cellular biology · 2009Article
- Splicing regulation: from a parts list of regulatory elements to an integrated splicing code.RNA (New York, N.Y.) · 2008Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Serine/arginine-rich (SR) proteins are essential splicing factors with one or two RNA-recognition motifs (RRMs) and a C-terminal arginine- and serine-rich (RS) domain. SR proteins bind to exonic splicing enhancers via their RRM(s), and from this position are thought to promote splicing by antagonizing splicing silencers, recruiting other components of the splicing machinery through RS-RS domain interactions, and/or promoting RNA base-pairing through their RS domains. An RS domain tethered at an exonic splicing enhancer can function as a splicing activator, and RS domains play prominent roles in current models of SR protein functions. However, we previously reported that the RS domain of the SR protein SF2/ASF is dispensable for in vitro splicing of some pre-mRNAs. We have now extended these findings via the identification of a short inhibitory domain at the SF2/ASF N-terminus; deletion of this segment permits splicing in the absence of this SR protein's RS domain of an IgM pre-mRNA substrate previously classified as RS-domain-dependent. Deletion of the N-terminal inhibitory domain increases the splicing activity of SF2/ASF lacking its RS domain, and enhances its ability to bind pre-mRNA. Splicing of the IgM pre-mRNA in S100 complementation with SF2/ASF lacking its RS domain still requires an exonic splicing enhancer, suggesting that an SR protein RS domain is not always required for ESE-dependent splicing activation. Our data provide additional evidence that the SF2/ASF RS domain is not strictly required for constitutive splicing in vitro, contrary to prevailing models for how the domains of SR proteins function to promote splicing.
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