ArticlePloS one2007
Identification of a guanine nucleotide exchange factor for Arf3, the yeast orthologue of mammalian Arf6.
Article in PloS one, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 20 citations in OpenAlex.
- Ancient complement and lineage-specific evolution of the Sec7 ARF GEF proteins in eukaryotes.Molecular biology of the cell · 2019Article
- Regulation of Arf activation occurs via distinct mechanisms at early and late Golgi compartments.Molecular biology of the cell · 2017Article
- Snf1/AMP-activated protein kinase activates Arf3p to promote invasive yeast growth via a non-canonical GEF domain.Nature communications · 2015Article
- Aspergillus nidulans Ambient pH Signaling Does Not Require Endocytosis.Eukaryotic cell · 2015Article
- Arf3p GTPase is a key regulator of Bud2p activation for invasive growth in Saccharomyces cerevisiae.Molecular biology of the cell · 2013Article
- Fission yeast ucp3 gene encodes a putative Arf6 GTPase-activating protein.Molecular biology reports · 2011Article
- ARF family G proteins and their regulators: roles in membrane transport, development and disease.Nature reviews. Molecular cell biology · 2011Review
- A cytohesin homolog in Dictyostelium amoebae.PloS one · 2010Article
- Functions of actin in endocytosis.Cellular and molecular life sciences : CMLS · 2009Review
- Yeast Arf3p modulates plasma membrane PtdIns(4,5)P2 levels to facilitate endocytosis.Traffic (Copenhagen, Denmark) · 2008Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Small G proteins of the Arf and Rab families are fundamental to the organisation and activity of intracellular membranes. One of the most well characterised of these G proteins is mammalian Arf6, a protein that participates in many cellular processes including endocytosis, actin remodelling and cell adhesion. Exchange of GDP for GTP on Arf6 is performed by a variety of guanine nucleotide exchange factors (GEFs), principally of the cytohesin (PSCD) and EFA6 (PSD) families. In this paper we describe the characterisation of a GEF for the yeast orthologue of Arf6, Arf3, which we have named Yel1 (yeast EFA6-like-1) using yeast genetics, fluorescence microscopy and in vitro nucleotide exchange assays. Yel1 appears structurally related to the EFA6 family of GEFs, having an N-terminal Sec7 domain and C-terminal PH and coiled-coil domains. We find that Yel1 is constitutively targeted to regions of polarised growth in yeast, where it co-localises with Arf3. Moreover the Sec7 domain of Yel1 is required for its membrane targeting and for that of Arf3. Finally we show that the isolated Yel1 Sec7 domain strongly stimulates nucleotide exchange activity specifically on Arf3 in vitro.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.