ArticlePloS one2007
Differential requirements for MCM proteins in DNA replication in Drosophila S2 cells.
Article in PloS one, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 39 citations in OpenAlex.
- A high-throughput screening identifies MCM chromatin loading inhibitors targeting cells with increased replication origins.iScience · 2024Article
- Selective role of the DNA helicase Mcm5 in BMP retrograde signaling during Drosophila neuronal differentiation.PLoS genetics · 2022Article
- In silico reconstitution of DNA replication. Lessons from single-molecule imaging and cryo-tomography applied to single-particle cryo-EM.Current opinion in structural biology · 2022Review
- Inhibition of MCM2 enhances the sensitivity of ovarian cancer cell to carboplatin.Molecular medicine reports · 2019Article
- Destabilization of the MiniChromosome Maintenance (MCM) complex modulates the cellular response to DNA double strand breaks.Cell cycle (Georgetown, Tex.) · 2018Article
- The Human Replicative Helicase, the CMG Complex, as a Target for Anti-cancer Therapy.Frontiers in molecular biosciences · 2018Review
- Quantitative Proteomics Reveals Dynamic Interactions of the Minichromosome Maintenance Complex (MCM) in the Cellular Response to Etoposide Induced DNA Damage.Molecular & cellular proteomics : MCP · 2015Article
- Dynamic loading and redistribution of the Mcm2-7 helicase complex through the cell cycle.The EMBO journal · 2015Article
- Developmental regulation of the Tetrahymena thermophila origin recognition complex.PLoS genetics · 2015Article
- Expression of minichromosome maintenance 8 in chronic myelogenous leukemia.International journal of clinical and experimental pathology · 2015Article
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- Mcm8 and Mcm9 form a dimeric complex in Xenopus laevis egg extract that is not essential for DNA replication initiation.Cell cycle (Georgetown, Tex.) · 2013Article
- The MCM8-MCM9 complex promotes RAD51 recruitment at DNA damage sites to facilitate homologous recombination.Molecular and cellular biology · 2013Article
- Emerging players in the initiation of eukaryotic DNA replication.Cell division · 2012Article
- Post-transcriptional homeostasis and regulation of MCM2-7 in mammalian cells.Nucleic acids research · 2012Article
- Role of replication protein A as sensor in activation of the S-phase checkpoint in Xenopus egg extracts.Nucleic acids research · 2012Article
- Drosophila RecQ4 is directly involved in both DNA replication and the response to UV damage in S2 cells.PloS one · 2012Article
- Depletion of cellular pre-replication complex factors results in increased human cytomegalovirus DNA replication.PloS one · 2012Article
- A genome-wide RNAi screen identifies core components of the G₂-M DNA damage checkpoint.Science signaling · 2011Article
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Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundThe MCM2-7 proteins are crucial components of the pre replication complex (preRC) in eukaryotes. Since they are significantly more abundant than other preRC components, we were interested in determining whether the entire cellular content was necessary for DNA replication in vivo. METHODOLOGY/PRINCIPLE
findingsWe performed a systematic depletion of the MCM proteins in Drosophila S2 cells using dsRNA-interference. Reducing MCM2-6 levels by >95-99% had no significant effect on cell cycle distribution or viability. Depletion of MCM7 however caused an S-phase arrest. MCM2-7 depletion produced no change in the number of replication forks as measured by PCNA loading. We also depleted MCM8. This caused a 30% reduction in fork number, but no significant effect on cell cycle distribution or viability. No additive effects were observed by co-depleting MCM8 and MCM5. CONCLUSIONS/SIGNIFICANCE: These studies suggest that, in agreement with what has previously been observed for Xenopus in vitro, not all of the cellular content of MCM2-6 proteins is needed for normal cell cycling. They also reveal an unexpected unique role for MCM7. Finally they suggest that MCM8 has a role in DNA replication in S2 cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.