ArticleMolecular and cellular biology2007
The Pu.1 locus is differentially regulated at the level of chromatin structure and noncoding transcription by alternate mechanisms at distinct developmental stages of hematopoiesis.
Article in Molecular and cellular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 64 citations in OpenAlex.
- JMJD1C forms condensate to facilitate a RUNX1-dependent gene expression program shared by multiple types of AML cells.Protein & cell · 2025Article
- Chromatin structure and 3D architecture define the differential functions of PU.1 regulatory elements in blood cell lineages.Epigenetics & chromatin · 2024Article
- Blood stem cell PU.1 upregulation is a consequence of differentiation without fast autoregulation.The Journal of experimental medicine · 2022Article
- Genomic and epigenomic adaptation in SP-R210 (Myo18A) isoform-deficient macrophages.Immunobiology · 2021Article
- Article
- Stage-specific action of Runx1 and GATA3 controls silencing of PU.1 expression in mouse pro-T cells.The Journal of experimental medicine · 2021Article
- Multi-scale Dynamical Modeling of T Cell Development from an Early Thymic Progenitor State to Lineage Commitment.Cell reports · 2021Article
- Dysregulation of NIPBL leads to impaired RUNX1 expression and haematopoietic defects.Journal of cellular and molecular medicine · 2020Article
- Runx1 promotes murine erythroid progenitor proliferation and inhibits differentiation by preventing Pu.1 downregulation.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Programming for T-lymphocyte fates: modularity and mechanisms.Genes & development · 2019Review
- Bcl11b and combinatorial resolution of cell fate in the T-cell gene regulatory network.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Dynamic Gene Regulatory Networks of Human Myeloid Differentiation.Cell systems · 2017Article
- RUNX1 induces DNA replication independent active DNA demethylation at SPI1 regulatory regions.BMC molecular biology · 2017Article
- Histone Chaperone HIRA in Regulation of Transcription Factor RUNX1.The Journal of biological chemistry · 2015Article
- The RUNX1-PU.1 axis in the control of hematopoiesis.International journal of hematology · 2015Review
- Myeloid-derived suppressor cells impair alveolar macrophages through PD-1 receptor ligation during Pneumocystis pneumonia.Infection and immunity · 2015Article
- Transcriptional Factor PU.1 Regulates Decidual C1q Expression in Early Pregnancy in Human.Frontiers in immunology · 2015Article
- Emerging and Novel Functions of Complement Protein C1q.Frontiers in immunology · 2015Review
- Optimization of transcription factor binding map accuracy utilizing knockout-mouse models.Nucleic acids research · 2014Article
- Cooperative interaction of Etv2 and Gata2 regulates the development of endothelial and hematopoietic lineages.Developmental biology · 2014Article
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Authors and funding
12 authors at 3 institutions in 2 countries.
Funding
Abstract
The Ets family transcription factor PU.1 is crucial for the regulation of hematopoietic development. Pu.1 is activated in hematopoietic stem cells and is expressed in mast cells, B cells, granulocytes, and macrophages but is switched off in T cells. Many of the transcription factors regulating Pu.1 have been identified, but little is known about how they organize Pu.1 chromatin in development. We analyzed the Pu.1 promoter and the upstream regulatory element (URE) using in vivo footprinting and chromatin immunoprecipitation assays. In B cells, Pu.1 was bound by a set of transcription factors different from that in myeloid cells and adopted alternative chromatin architectures. In T cells, Pu.1 chromatin at the URE was open and the same transcription factor binding sites were occupied as in B cells. The transcription factor RUNX1 was bound to the URE in precursor cells, but binding was down-regulated in maturing cells. In PU.1 knockout precursor cells, the Ets factor Fli-1 compensated for the lack of PU.1, and both proteins could occupy a subset of Pu.1 cis elements in PU.1-expressing cells. In addition, we identified novel URE-derived noncoding transcripts subject to tissue-specific regulation. Our results provide important insights into how overlapping, but different, sets of transcription factors program tissue-specific chromatin structures in the hematopoietic system.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.