ArticleMolecular and cellular biology2007
Restricting conformational flexibility of the switch II region creates a dominant-inhibitory phenotype in Obg GTPase Nog1.
Article in Molecular and cellular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 22 citations in OpenAlex.
- The 3'-5' exoribonuclease ISG20L2 contributes to 3' terminus maturation of 18S and 28S ribosomal RNAs.Nucleic acids research · 2025Article
- Mutational Analysis of the Nsa2 N-Terminus Reveals Its Essential Role in Ribosomal 60S Subunit Assembly.International journal of molecular sciences · 2020Article
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- The Conserved RNA Exonuclease Rexo5 Is Required for 3' End Maturation of 28S rRNA, 5S rRNA, and snoRNAs.Cell reports · 2017Article
- Principles of 60S ribosomal subunit assembly emerging from recent studies in yeast.The Biochemical journal · 2017Review
- What maintains the high intra-follicular estradiol concentration in pre-ovulatory follicles?Journal of assisted reproduction and genetics · 2016Article
- Human nucleolar protein Nop52 (RRP1/NNP-1) is involved in site 2 cleavage in internal transcribed spacer 1 of pre-rRNAs at early stages of ribosome biogenesis.Nucleic acids research · 2015Article
- Coupled GTPase and remodelling ATPase activities form a checkpoint for ribosome export.Nature · 2014Article
- Two orthogonal cleavages separate subunit RNAs in mouse ribosome biogenesis.Nucleic acids research · 2014Article
- Nucleolar GTPase NOG-1 regulates development, fat storage, and longevity through insulin/IGF signaling in C. elegans.Molecules and cells · 2014Article
- 5'-end surveillance by Xrn2 acts as a shared mechanism for mammalian pre-rRNA maturation and decay.Nucleic acids research · 2011Article
- Biochemical and physiological characterization of the GTP-binding protein Obg of Mycobacterium tuberculosis.BMC microbiology · 2011Article
- Mammalian DEAD box protein Ddx51 acts in 3' end maturation of 28S rRNA by promoting the release of U8 snoRNA.Molecular and cellular biology · 2010Article
- A genome-scale protein interaction profile of Drosophila p53 uncovers additional nodes of the human p53 network.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Saccharomyces cerevisiae Rbg1 protein and its binding partner Gir2 interact on Polyribosomes with Gcn1.Eukaryotic cell · 2009Article
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4 authors at 1 institution in 1 country.
Funding
Abstract
Nog1 is a conserved eukaryotic GTPase of the Obg family involved in the biogenesis of 60S ribosomal subunits. Here we report the unique dominant-inhibitory properties of a point mutation in the switch II region of mouse Nog1; this mutation is predicted to restrict conformational mobility of the GTP-binding domain. We show that although the mutation does not significantly affect GTP binding, ectopic expression of the mutant in mouse cells disrupts productive assembly of pre-60S subunits and arrests cell proliferation. The mutant impairs processing of multiple pre-rRNA intermediates, resulting in the degradation of the newly synthesized 5.8S/28S rRNA precursors. Sedimentation analysis of nucleolar preribosomes indicates that defective Nog1 function inhibits the conversion of 32S pre-rRNA-containing complexes to a smaller form, resulting in a drastic accumulation of enlarged pre-60S particles in the nucleolus. These results suggest that conformational changes in the switch II element of Nog1 have a critical importance for the dissociation of preribosome-bound factors during intranucleolar maturation and thereby strongly influence the overall efficiency of the assembly process.
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