ArticleEukaryotic cell2007
Hydrogen production by termite gut protists: characterization of iron hydrogenases of Parabasalian symbionts of the termite Coptotermes formosanus.
Article in Eukaryotic cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 51 citations in OpenAlex.
- Impact of Wood Age on Termite Microbial Assemblages.Applied and environmental microbiology · 2023Article
- Article
- Termite gas emissions select for hydrogenotrophic microbial communities in termite mounds.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Methanogenesis in the Digestive Tracts of the Tropical Millipedes Archispirostreptus gigas (Diplopoda, Spirostreptidae) and Epibolus pulchripes (Diplopoda, Pachybolidae).Applied and environmental microbiology · 2021Article
- Molecular characterization of Trichomonas gypaetinii isolated from the upper alimentary tract of Steller's sea eagles (Haliaeetus pelagicus) and white-tailed sea eagles (Haliaeetus albicilla) in Hokkaido, Japan.Parasitology research · 2021Article
- Division of functional roles for termite gut protists revealed by single-cell transcriptomes.The ISME journal · 2020Article
- Parallel reductive genome evolution in Desulfovibrio ectosymbionts independently acquired by Trichonympha protists in the termite gut.The ISME journal · 2020Article
- Heterologous Hydrogenase Overproduction Systems for Biotechnology-An Overview.International journal of molecular sciences · 2020Review
- Article
- Genome analyses of uncultured TG2/ZB3 bacteria in 'Margulisbacteria' specifically attached to ectosymbiotic spirochetes of protists in the termite gut.The ISME journal · 2019Article
- Article
- Genome of 'Ca. Desulfovibrio trichonymphae', an HThe ISME journal · 2017Article
- Lower Termite Associations with Microbes: Synergy, Protection, and Interplay.Frontiers in microbiology · 2016Review
- Metabolomic profiling of 13C-labelled cellulose digestion in a lower termite: insights into gut symbiont function.Proceedings. Biological sciences · 2014Article
- Symbiotic digestion of lignocellulose in termite guts.Nature reviews. Microbiology · 2014Review
- Comprehensive phylogenetic diversity of [FeFe]-hydrogenase genes in termite gut microbiota.Microbes and environments · 2013Article
- Patterns of [FeFe] hydrogenase diversity in the gut microbial communities of lignocellulose-feeding higher termites.Applied and environmental microbiology · 2012Article
- Bacteroidales ectosymbionts of gut flagellates shape the nitrogen-fixing community in dry-wood termites.The ISME journal · 2012Article
- Analysis of extensive [FeFe] hydrogenase gene diversity within the gut microbiota of insects representing five families of Dictyoptera.Microbial ecology · 2012Article
- Genomic analysis reveals multiple [FeFe] hydrogenases and hydrogen sensors encoded by treponemes from the H(2)-rich termite gut.Microbial ecology · 2012Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cellulolytic flagellated protists in the guts of termites produce molecular hydrogen (H(2)) that is emitted by the termites; however, little is known about the physiology and biochemistry of H(2) production from cellulose in the gut symbiotic protists due to their formidable unculturability. In order to understand the molecular basis for H(2) production, we here identified two genes encoding proteins homologous to iron-only hydrogenases (Fe hydrogenases) in Pseudotrichonympha grassii, a large cellulolytic symbiont in the phylum Parabasalia, in the gut of the termite Coptotermes formosanus. The two Fe hydrogenases were phylogenetically distinct and had different N-terminal accessory domains. The long-form protein represented a phylogenetic lineage unique among eukaryotic Fe hydrogenases, whereas the short form was monophyletic with those of other parabasalids. Active recombinant enzyme forms of these two Fe hydrogenases were successfully obtained without the specific auxiliary maturases. Although they differed in their extent of specific activity and optimal pH, both enzymes preferentially catalyzed H(2) evolution rather than H(2) uptake. H(2) evolution, at least that associated with the short-form enzyme, was still active even under high hydrogen partial pressure. H(2) evolution activity was detected in the hydrogenosomal fraction of P. grassii cells; however, the vigorous H(2) uptake activity of the endosymbiotic bacteria compensated for the strong H(2) evolution activity of the host protists. The results suggest that termite gut symbionts are a rich reservoir of novel Fe hydrogenases whose properties are adapted to the gut environment and that the potential of H(2) production in termite guts has been largely underestimated.
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