ArticleEukaryotic cell2008
Fully codon-optimized luciferase uncovers novel temperature characteristics of the Neurospora clock.
Article in Eukaryotic cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 100 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
100 citing papers in PubMed, 151 citations in OpenAlex.
- Core clock protein subcellular dynamics coordinate local and global circadian control in syncytia.The Journal of cell biology · 2026Article
- A daily cycle of White Collar Complex dephosphorylation sustains circadian rhythmicity inProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Core Clock Protein Subcellular Dynamics Coordinate Local and Global Circadian Control in Syncytia.bioRxiv : the preprint server for biology · 2025Article
- Individual peroxiredoxin or Tor pathway components are not required for circadian clock function in Neurospora crassa.Fungal biology · 2025Article
- Paf1C regulates the Neurospora circadian clock by promoting the transcription elongation of frequency.PLoS genetics · 2025Article
- DNA Sequence Changes Resulting from Codon Optimization Affect Gene Expression inJournal of fungi (Basel, Switzerland) · 2025Article
- Circadian clock is critical for fungal pathogenesis by regulating zinc starvation response and secondary metabolism.Science advances · 2025Article
- Checkpoint kinases regulate the circadian clock after DNA damage by influencing chromatin dynamics.Nucleic acids research · 2025Article
- Circadian rhythms: pervasive, and often times evasive.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- Origin of the clock inFrontiers in molecular biosciences · 2025Review
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- Engineering of Aspergillus niger for efficient production of D-xylitol from L-arabinose.Microbial cell factories · 2024Article
- A novel luciferase-based reporter tool to monitor the dynamics of carbon catabolite repression in filamentous fungi.Microbial biotechnology · 2024Article
- The clock in growing hyphae and their synchronization in Neurospora crassa.Communications biology · 2024Article
- The auxotrophicFungal genetics reports · 2024Article
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- Antisense Transcription of theJournal of biological rhythms · 2023Article
- Conformational changes in the negative arm of the circadian clock correlate with dynamic interactomes involved in post-transcriptional regulation.Cell reports · 2023Article
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40 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
We report the complete reconstruction of the firefly luciferase gene, fully codon optimized for expression in Neurospora crassa. This reporter enhances light output by approximately 4 log orders over that with previously available versions, now producing light that is visible to the naked eye and sufficient for monitoring the activities of many poorly expressed genes. Time lapse photography of strains growing in race tubes, in which the frq or eas/ccg-2 promoter is used to drive luciferase, shows the highest levels of luciferase activity near the growth front and newly formed conidial bands. Further, we have established a sorbose medium colony assay that will facilitate luciferase-based screens. The signals from sorbose-grown colonies of strains in which the frq promoter drives luciferase exhibit the properties of circadian rhythms and can be tracked for many days to weeks. This reporter now makes it possible to follow the clock in real time, even in strains or under conditions in which the circadian rhythm in conidial banding is not expressed. This property has been used to discover short, ca. 15-h period rhythms at high temperatures, at which banding becomes difficult to observe in race tubes, and to generate a high-resolution temperature phase-response curve.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.