ArticleApplied and environmental microbiology2007
Damage in Escherichia coli cells treated with a combination of high hydrostatic pressure and subzero temperature.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 86 citations in OpenAlex.
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- Synergistic effect of high-pressure thermal sterilization and muramidase on Bacillus subtilis spores: alterations in intrasporal components, inner membrane permeability, and structural integrity.Archives of microbiology · 2025Article
- N-terminus GTPase domain of the cytoskeleton protein FtsZ plays a critical role in its adaptation to high hydrostatic pressure.Frontiers in microbiology · 2024Article
- Unraveling the Antibacterial Mechanism of Plasma-Activated Lactic Acid againstFoods (Basel, Switzerland) · 2023Article
- Recent Progress in the Synergistic Bactericidal Effect of High Pressure and Temperature Processing in Fruits and Vegetables and Related Kinetics.Foods (Basel, Switzerland) · 2022Review
- High-Pressure-Induced Sublethal Injuries of Food Pathogens-Microscopic Assessment.Foods (Basel, Switzerland) · 2021Article
- Microbial inactivation by high pressure processing: principle, mechanism and factors responsible.Food science and biotechnology · 2021Review
- Synergistic Effects of Bacteriocin fromAntibiotics (Basel, Switzerland) · 2020Article
- The Polyextremophilic Bacterium Clostridium paradoxum Attains Piezophilic Traits by Modulating Its Energy Metabolism and Cell Membrane Composition.Applied and environmental microbiology · 2019Article
- Antibacterial Activity ofFrontiers in cellular and infection microbiology · 2019Article
- Potentiating the Heat Inactivation of Escherichia coli O157:H7 in Ground Beef Patties by Natural Antimicrobials.Frontiers in microbiology · 2016Article
- Article
- Microscopic analysis of bacterial motility at high pressure.Biophysical journal · 2012Article
- Bacterial motility measured by a miniature chamber for high-pressure microscopy.International journal of molecular sciences · 2012Article
- Comprehensive characterization of cell and tissue responses toward high hydrostatic pressure treatment: Molecular feedback and structural integrity in bone graft processing.Journal of tissue engineeringArticle
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The relationship between membrane permeability, changes in ultrastructure, and inactivation in Escherichia coli strain K-12TG1 cells subjected to high hydrostatic pressure treatment at room and subzero temperatures was studied. Propidium iodide staining performed before and after pressure treatment made it possible to distinguish between reversible and irreversible pressure-mediated cell membrane permeabilization. Changes in cell ultrastructure were studied using transmission electron microscopy (TEM), which showed noticeable condensation of nucleoids and aggregation of cytosolic proteins in cells fixed after decompression. A novel technique used to mix fixation reagents with the cell suspension in situ under high hydrostatic pressure (HHP) and subzero-temperature conditions made it possible to show the partial reversibility of pressure-induced nucleoid condensation. However, based on visual examination of TEM micrographs, protein aggregation did not seem to be reversible. Reversible cell membrane permeabilization was noticeable, particularly for HHP treatments at subzero temperature. A correlation between membrane permeabilization and cell inactivation was established, suggesting different mechanisms at room and subzero temperatures. We propose that the inactivation of E. coli cells under combined HHP and subzero temperature occurs mainly during their transiently permeabilized state, whereas HHP inactivation at room temperature is related to a balance of transient and permanent permeabilization. The correlation between TEM results and cell inactivation was not absolute. Further work is required to elucidate the effects of pressure-induced damage on nucleoids and proteins during cell inactivation.
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