ArticlePlant physiology2007
The import of S-adenosylmethionine into the Golgi apparatus is required for the methylation of homogalacturonan.
Article in Plant physiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 39 citations in OpenAlex.
- Pectin Structural Dynamics: Developmental and Evolutionary Perspectives.Plants (Basel, Switzerland) · 2026Review
- Pectin under stress: Pectin methylesterases at the core of cell wall remodeling, integrity sensing, and signaling in plants.Plant communications · 2026Review
- CmbZIP11 regulatesHorticulture research · 2026Article
- PME10 Is a Pectin Methylesterase Driving PME Activity and Immunity Against Botrytis cinerea in Grapevine (Vitis vinifera L.).Plant biotechnology journal · 2025Article
- Pectin Metabolism Influences Phloem Architecture and Flowering Time in Arabidopsis Thaliana.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Different strategies of altering the cell wall to study the impact that these introduced changes have on plant resistance to pathogens.Frontiers in plant science · 2025Review
- Deepening the Role of Pectin in the Tissue Assembly Process During Tomato Grafting.Plants (Basel, Switzerland) · 2024Article
- Pull the fuzes: Processing protein precursors to generate apoplastic danger signals for triggering plant immunity.Plant communications · 2024Review
- Article
- Golgi-localized putative S-adenosyl methionine transporters required for plant cell wall polysaccharide methylation.Nature plants · 2022Article
- Protoplast isolation and transcriptome analysis of developing xylem in Pinus massoniana (Pinaceae).Molecular biology reports · 2022Article
- AtPME17 is a functional Arabidopsis thaliana pectin methylesterase regulated by its PRO region that triggers PME activity in the resistance to Botrytis cinerea.Molecular plant pathology · 2020Article
- Mutations in the Pectin Methyltransferase QUASIMODO2 Influence Cellulose Biosynthesis and Wall Integrity in Arabidopsis.The Plant cell · 2020Article
- Influence of cell wall polymers and their modifying enzymes during plant-aphid interactions.Journal of experimental botany · 2020Review
- Pectin Methylesterases Modulate Plant Homogalacturonan Status in Defenses against the AphidThe Plant cell · 2019Article
- Xylan in the Middle: Understanding Xylan Biosynthesis and Its Metabolic Dependencies Toward Improving Wood Fiber for Industrial Processing.Frontiers in plant science · 2019Review
- Pectin and Pectin-Based Composite Materials: Beyond Food Texture.Molecules (Basel, Switzerland) · 2018Review
- Rice putative methyltransferase gene OsTSD2 is required for root development involving pectin modification.Journal of experimental botany · 2016Article
- PRMT1-mediated methylation of the EGF receptor regulates signaling and cetuximab response.The Journal of clinical investigation · 2015Article
- Methylated glycans as conserved targets of animal and fungal innate defense.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
S-adenosylmethionine (SAM) is the substrate used in the methylation of homogalacturonan (HGA) in the Golgi apparatus. SAM is synthesized in the cytosol, but it is not currently known how it is then transported into the Golgi. In this study, we find that HGA methyltransferase is present in Golgi-enriched fractions and that its catalytic domain faces the lumen of this organelle. This suggests that SAM must be imported into the Golgi. We performed uptake experiments using [methyl-(14)C]SAM and found that SAM is incorporated into the Golgi vesicles, resulting in the methylation of polymers that are sensitive to pectinase and pectin methylesterase but not to proteases. To avoid detecting the transfer reaction, we also used [carboxyl-(14)C]SAM, the uptake of which into Golgi vesicles was found to be sensitive to temperature, detergents, and osmotic changes, and to be saturable with a K(m) of 33 microm. Double-label uptake experiments using [methyl-(3)H]SAM and [carboxyl-(14)C]SAM also revealed a time-dependent increase in the (3)H to (14)C ratio, suggesting that upon transfer of the methyl group, the resulting S-adenosylhomocysteine is not accumulated in the Golgi. SAM incorporation was also found to be inhibited by S-adenosylhomocysteine, whereas UDP-GalA, UDP-GlcA, and acetyl-CoA had no effect. DIDS, a compound that inhibits nucleotide sugar transporters, also had little effect upon SAM incorporation. Interestingly, the combination of UDP-GalA + acetyl-CoA or UDP-GlcA + acetyl-CoA produced a slight increase in the uptake of SAM. These results support the idea that a SAM transporter is required for HGA biosynthesis.
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Registered trials
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