ArticleBiophysical journal2008
Phase fluctuations on the micron-submicron scale in GUVs composed of a binary lipid mixture.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- LAURDAN since Weber: The Quest for Visualizing Membrane Heterogeneity.Accounts of chemical research · 2021Article
- Laurdan generalized polarization fluctuations measures membrane packing micro-heterogeneity in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Precise and millidegree stable temperature control for fluorescence imaging: application to phase transitions in lipid membranes.The Review of scientific instruments · 2010Article
- Lipid packing determines protein-membrane interactions: challenges for apolipoprotein A-I and high density lipoproteins.Biochimica et biophysica acta · 2010Review
- Dynamics of lipid domain formation: fluctuation analysis.Biochimica et biophysica acta · 2010Review
- Site-directed fluorescence labeling of a membrane protein with BADAN: probing protein topology and local environment.Biophysical journal · 2008Article
- Phase diagrams of lipid mixtures relevant to the study of membrane rafts.Biochimica et biophysica actaReview
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
We used a combination of imaging and fluctuation techniques to investigate the temporal evolution of gel phase domains at the onset of phase separation, as well as the correlation between domain topology and local lipid ordering in GUVs composed of a binary mixture of DPPC/DLPC 1:1. The data acquired at temperatures immediately above the transition temperature of the two lipids suggest fluctuations in the lipid organization with a lifetime <0.1 s and a characteristic length of 1.2 microm. As the temperature is decreased below the transition temperature of one of the lipids, coupling between the two leaflets of the bilayer is observed to begin within the first five minutes after the onset of phase separation. However, domains confined to only one leaflet can be found during the first 45-50 min after the onset of phase separation. Our analysis using a two-state model (liquid and gel) indicates that for the first 45-50 min from the onset of phase separation the two lipid phases do not strongly influence the phase behavior of each other on the micron-length scale. At longer times, behavior that deviates from the two-state model is observed and appears to be correlated to domain morphology.
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Registered trials
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